Molecular Genetics and Genetic Engineering
Samaneh Bagheri; Barat-Ali Fakheri; Ali Mohammad Ahadi; Abbasali Emamjomeh
Abstract
Wheat streak mosaic virus (WSMV) is one of the most important wheat infectious which its prevalence is increasing in Iran. NIa protein as a key protein in WSMV, plays important roles in viral replication and proteolytic digestion of viral polyprotein. Considering the critical role of NIa protein in viral ...
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Wheat streak mosaic virus (WSMV) is one of the most important wheat infectious which its prevalence is increasing in Iran. NIa protein as a key protein in WSMV, plays important roles in viral replication and proteolytic digestion of viral polyprotein. Considering the critical role of NIa protein in viral infection, isolation, sequencing and determination of secondary and tertiary structure of this protein were the objectives of the present study. Also, the amino acids present in the NIa protein active site, which can be used as the targets for design and developement of new antiviral agents, were investigated. In this study, the gene encoding NIa protein was isolated from WSMV and its sequencing was done followed by cloning in pEST prokaryotic vector. The resulted nucleotide sequence was deposited in NCBI database with accession number MK335432. Investigation of physical and chemical properties showed that NIa protein is including 426 amino acids, 48.8 kD molecular weight and 8.81 PI. Prediction of secondary structure of NIa protein revealed that 53.29% of its structure composed of irregular loops, which was justified by the structural dirsorder of this protein. The results of the active site investigation of NIa protein based on homologous sequences alignment and three-dimensional structure of the protein showed a highly conserved site which was included of histidine, aspartic acid and cysteine amino acids.
Molecular Plant Breeding
Maliheh Shahraki; Abbasali Emamjomeh; Baratali Fakheri; Bahman Fazeli-Nasab
Abstract
To evaluate genetic diversity between Sistan common wheat cultivars, it was used 10 SSRs primers associated to stem, leaf and yellow rusts resistance genes. The lowest (3) and highest (7) allele number were generated by 12C, SCS719 and Xgdm116 primers and Xgwm443 primer, respectively (4.55 allele per ...
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To evaluate genetic diversity between Sistan common wheat cultivars, it was used 10 SSRs primers associated to stem, leaf and yellow rusts resistance genes. The lowest (3) and highest (7) allele number were generated by 12C, SCS719 and Xgdm116 primers and Xgwm443 primer, respectively (4.55 allele per each primer). The highest genetic diversity (0.39) and MI (2.29) was related to Xgdm116 and Xgwm533 primers, respectively; also, the lowest genetic diversity (0.1) and MI (0.33) was related to Xcfd36 primer. Xgdm36 primer showed the highest Ne, Shannon diversity and Nei diversity (1.45, 0.63 and 0.44, respectively); on the other hand, Xcfd36 primer the lowest Ne, Shannon diversity and Nei diversity (1.18, 0.19 and 0.11, respectively). The highest polymorphic bands between wheat cultivars were related to Arg. Arg and Aflak showed the least diversity for resistance to yellow rust and leaf rust, respectively. Also, Arg had the highest diversity for three types of rusts. The Xgdm116, Xwmc810 and SCS719 primers had more effect on identification of wheat cultivars. Finally, Arg cultivar can be recommended as a donor parent in wheat breeding programs for rust resistance. To gain the highest heterosis, it can be suggested hybridization between Arg and Hirmand cultivars.
