با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد بیوتکنولوی کشاورزی، دانشگاه زابل، زابل،

2 دانشیار گروه بیوتکنولوژی کشاورزی، دانشگاه زابل، زابل

3 مربی پژوهشکده زیست فناوری دانشگاه زابل، زابل

چکیده

گیاه Ocimum basilicum L.  از خانواده نعناعیان است که دارای ترکیبات حلقوی و اسانس بوده و خواص ضد باکتریایی و آنتی‌اکسیدانی دارد، که سرشار از ترکیبات فنیل پروپانوئیدی است. آنزیم فنیل‌آلانین آمونیالیاز یکی از آنزیم­های کلیدی در مسیر تولید ترکیبات فنیل پروپانوئیدی می­باشد. شواهد نشان می­دهد که هورمون­های گیاهی بر بیان ژن در گیاهان مؤثر بوده و موجب افزایش تولید متابولیت­های ثانویه می‏گردند. علاوه بر این سیستم دفاعی گیاه را از طریق فعال‏سازی رونویسی ژن­های مرتبط با مکانیسم دفاعی گیاه تحریک نموده باعث تنظیم مقاومت القایی می­گردند. در این تحقیق، بیان ژن فنیل‌آلانین‌آمونیالیاز و فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز در گیاه ریحان تحت تیمار هورمون­های اسید جیبرلیک، اسید جاسمونیک و اسید سالیسیلیک با غلظت 1/0 میلی‏مولار در لیتر با روش Real Time PCR در مراحل رشدی گیاهچه­ای، پیش­گلدهی و گلدهی مورد بررسی قرار گرفت. اختلاف سطوح بیان ژن با روش دانکن در سطح 05/0P≤ مقایسه و آنالیز­ها توسط نرم افزار SAS v9 صورت گرفت. نتایج نشان دهنده افزایش در میزان بیان ژن و فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز در تیمار هورمون اسید جاسمونیک در مرحله گلدهی بود. بیان ژن فنیل‌آلانین‌آمونیالیاز بین تیمار هورمون­های اسید جیبرلیک، اسید جاسمونیک و اسید سالیسیلیک و نمونه شاهد در سطح احتمال 5 درصد معنا­دار بود. تیمار اسید جاسمونیک، اسید جیبرلیک و اسید سالیسیلیک باعث افزایش بیان ژن و فعالیت آنزیم فنیل‌آلانین‌آمونیالیاز شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Different Growth Hormones on Gene Expression of Phenylalanine Ammonia Lyase (PAL) in Ocimum basilicum L.

نویسندگان [English]

  • Sara Abdekhani 1
  • Mahmood Solouki 2
  • Yasub Shiri 3

1 M.Sc. of Agricultural Biotechnology, University of Zabol, Zabol, Iran

2 Associate Professor, Department of Biotechnology, University of Zabol, Zabol, Iran

3 Lecturer, Biotechnology Research Institute of University of Zabol. Zabol, Iran.

چکیده [English]

Basil (Ocimum basilicum L.) is a member of a Lamiaceae family with aroma compounds and high essence. Furthermore, the essential oils of basil leaves are composed of phenylpropanoids, which have shown antibacterial and antioxidant properties. Phenylalanine ammonia Lyase is an important enzyme in phenylpropanoid pathway. The literature indicated that plant hormones affect gene expression in plants and increase the production of secondary metabolites. In addition, the hormones stimulate the immune system through transcriptional activation of defense related genes, and in turns, increase induced resistance of plants. In the current study, Real Time PCR was used to evaluate the gene expression and enzymatic activity of phenylalanine ammonia. The plants were treated with three different hormones including gibberellic acid, jasmonic acid, and salicylic acid with 0.1 mM/L concentration. The activities were studied during three different plant growth stages which included seedlings, pre-flowering stage, and flowering stage. The difference between gene expressions levels were analyzed by Duncan method (P≤0.05) using SAS software (version 9.0). During the flowering stage, the results showed an increase in gene expression of phenylalanine ammonia Lyase in plants treated with jasmonic acid. The gene expression of phenylalanine ammonia Lyase was significantly higher (5%) for all treated samples compared to the control samples. The studied hormones increased the gene expression and enzymatic activity of phenylalanine ammonia Lyase. 

کلیدواژه‌ها [English]

  • phenylalanine ammonia lyase
  • hormone
  • RT-PCR
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