با همکاری مشترک دانشگاه پیام نور و انجمن بیوتکنولوژی جمهوری اسلامی ایران

نوع مقاله : علمی پژوهشی

نویسندگان

1 دانشجوی دکتری گروه بیماری‌شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

2 استادیار گروه زیست فراورده‌های گیاهی، پژوهشکده زیست‌فناوری کشاورزی، پژوهشگاه ملی مهندسی ژنتیک و زیست‌فناوری، تهران، ایران

3 استاد گروه بیماری شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

4 دانشیار گروه بیماری شناسی گیاهی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران.

چکیده

القاء مقاومت به بیمارگرها از جمله راه‌کارهای مقابله با تنش‌های زیستی در گیاهان می‌باشد. آنزیم‌های کیتیناز و بتا 1و3 گلوکوناز از جمله پروتئین-های دفاعی قابل‌القاء هستند که در پاسخ به‎عوامل بیماریزا توسط گیاه، تولید می‌شوند. در این تحقیق، در ابتدا تاثیر تیمار اسیدسالیسیلیک و باکتری Pseudomonas flourescens در کاهش علایم بیماری سوختگی برگ گندم مورد بررسی قرار گرفت. سپس تغییرات بیان ژن‌های کیتیناز و بتا 1و3 گلوکوناز در نمونه‌های گیاهی تیمار شده و کنترل در روزهای 0، 4، 14 و 24 سیکل بیماری به‎روش RT-PCR نیمه کمی بررسی شدند. نتایج نشان‌دهندۀ افزایش بیان هر دو ژن در روز چهارم پس از تلقیح بیمارگر بود و کاربرد اسیدسالیسیک و باکتری بیوکنترل به‎صورت جداگانه توانست بیان آن‌ها را افزایش دهد، ولی کاربرد همزمان هر دو تیمار، کاهش معنی‌داری از نظر آماری ایجاد نکرد. بر اساس نتایج، افزایش بیان آنزیم‌های کیتیناز و بتا-1و3 گلوکوناز با کاربرد اسیدسالیسیک و باکتری بیوکنترل P. flourescens می‌تواند در القاء مقاومت میزبانی در مقابله با بیماری سوختگی برگ گندم نقش داشته باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of chitinase and β-1,3glucanase expression profile in Pseudomonas flourescens and salicylic acid treated wheat against Septoria tritici bloch (STB)

نویسندگان [English]

  • Elham Zamani 1
  • Forogh Sanjarian 2
  • Ebrahim Mohammadi-goltapeh 3
  • Naser Safaie 4

1 Ph.D. student, Department of Plant Pathology, Tarbiat Modares University, Faculty of Agriculture, Tehran, Iran

2 Assistant Professor, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

3 Professor, Department of Plant Pathology, Tarbiat Modares University, Faculty of Agriculture, Tehran, Iran

4 Associate Professor, Department of Plant Pathology, Tarbiat Modares University, Faculty of Agriculture, Tehran, Iran

چکیده [English]

Induced resistance is one the ways by which plants cope with the biotic stresses. Pathogenesis-related proteins (PRs), including chitinase and β-1,3-glucanase are synthesized by plant in response to pathogenic infection. In present work, we have carried out inoculation experiment in wheat to investigate the effect of salicylic acid (SA) and Pseudomonas flourescens on Septoria tritici Bloch (STB) symptoms. Subsequently, the expression profiles of chitinase and β-1,3-glucanase were analyzed with semi-quantitative RT-PCR at 0, 4, 14 and 24 days after inoculation in both treated and control plants. Results showed that the expression of both chitinase and β-1,3-glucanase genes increased in interaction with Z. tritici and the application of SA and P. flourescens. On the other hand, the application together had no significant effect on gene expression. Briefly, it can be concluded that increment of chitinase and β-1,3-glucanase expression level with the application of SA and P. flourescens would play role induction of plant defense against STB.

کلیدواژه‌ها [English]

  • “Salicylic acid
  • ” Pseudomonas flourescens
  • “Chitinase
  • “ Glucanase
  • “Septoria tritici bloch
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