بررسی نیمه کمی بیان ژن بتائین آلدئید دهیدروژناز در گیاه سیب‌زمینی (Solanum tuberosum cv. Agria) تحت تنش شوری

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

2 دانشیار گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

3 دانشیار گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران

4 دانشیار گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.

5 استادیار گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران

چکیده

این مطالعه با هدف بررسی نقش نیتریک‌اکسید بر میزان بیان ژن بتائین آلدئیددهیدروژناز و سنتز گلایسین‌بتائین در سیب‌زمینی رقم آگریا تحت تنش شوری در شرایط درون شیشه‌ای انجام شد. تیمار‌های آزمایش شامل چهار سطح سدیم‌نیترو پروساید به عنوان دهنده نیتریک‌اکسید (0، 3-10، 4-10 و 5-10 میلی‌مولار) و دو سطح سدیم‌کلرید (0 و 70 میلی‌مولار) بود. در این تحقیق از محیط کشت MS استفاده گردید و پس از اعمال تنش شوری از سدیم‌نیترو پروساید جهت تیمار ریزنمونه‌ها به منظور افزایش احتمالی بیان ژن بتائین آلدئید دهیدروژناز (ژن مسئول سنتز گلایسین‌بتائین) استفاده شد. چهار هفته بعد از اعمال تیمار، RNA کل از بافت‌های نمونه‌های تیمار شده استخراج شد و به منظور ارزیابی نسبی بیان ژن بتائین آلدئید دهیدروژناز از روش RT-PCR نیمه‌کمی استفاده گردید. بررسی بیان ژن بتائین آلدئید دهیدروژناز نشان داد که میزان بیان این ژن در گیاهچه‌های تحت تنش شوری افزایش داشته، در حالی‌که تیمار سدیم‌نیترو پروساید در شرایط تنش شوری میزان بیان آن را کاهش داد. همچنین میزان گلایسین‌بتائین در بافت‌های نمونه‌های گیاهی رشد یافته در شرایط معمولی با به کار بردن سدیم‌نیترو پروساید افزایش نشان داد، درحالی‌که سدیم‌نیتروپروساید تأثیر منفی روی محتوای گلایسین‌بتائین گیاهچه‌ها تحت شرایط تنش شوری داشته است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Semi quantitative analysis of betaine aldehyde dehydrogenase gene expression in potato plant under salinity stress

نویسندگان [English]

  • Zhila Mohammadi 1
  • Alireza Motallebi-Azar 2
  • Fariborz Zaree-Nahandi 3
  • Alireza Tarinejad 4
  • Gholamreza Gohari 5
1 Former M.Sc. Student of Horticulture, Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.
2 Associate Professor, Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.
3 Associate Professor, Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.
4 Assistant Professor, Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.
5 Assistant Professor, Department of Horticulture, Faculty Agriculture, University of Maragheh, Maragheh, Iran.
چکیده [English]

The present study was aimed to investigate of nitric oxide effect on betaine aldehyde dehydrogenase gene expression and glycine betaine synthesis in Solanum tuberosum cv. Agria under salinity stress on in vitro condition. The experiment treatments included four level of sodium nitroprussideas a nitric oxide donor (0, 10-3, 10-4 and 10-5mM) and two level of sodium chloride (0 and 70 mM). In the present study, MS media culture was used and sodium nitroprusside was applied for increasing the betaine aldehyde dehydrogenase gene expression (the responsible gene of glycine betaine synthesis) under salinity stress. Four weeks after treatment, total RNA of treated explants was extracted and semi quantitative RT-PCR was used for the analysis of expression of betaine aldehyde dehydrogenase gene. The glycinbetaine content was measured with iodide potassium. The survey of betaine aldehyde dehydrogenase gene expression showed that the expression of this gene was increased under salinity stress however, the sodium nitroprusssid decreased its expression under salinity stress. Also sodium nitroprussid increased the glycine betaine content in grown plantlets which were grown under normal condition however under salinity stress this compound showed negative effect on glycinbetaine content.

کلیدواژه‌ها [English]

  • salinity stress
  • glycinbetaine
  • betaine aldehyde dehydrogenase
  • Gene expression
  • semi quantitative RT-PCR
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