مطالعه تغییرات مورفو-فیزیولوژی و الگوی پروتئینی ارقام متحمل و حساس گندم تحت تنش شوری با استفاده از تجزیه پروتئوم

نوع مقاله: علمی پژوهشی

نویسندگان

1 استادیار بخش کشاورزی، دانشگاه پیام نور، تهران، ایران

2 دانشیار بخش کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

به منظور بررسی مکانسیم تحمل به تنش شوری در ارقام گندم، آزمایشی فاکتوریل بر پایه طرح کاملاً تصادفی با چهار تکرار در محیط گلخانه انجام شد. عوامل این آزمایش شامل تنش شوری از نوع کلریدسدیم در چهار سطح صفر (شاهد)، 100، 200 و 300 میلی‌مول و دو رقم گندم نیک‌نژاد و پیشتاز بترتیب بعنوان نماینده‌ای از ارقام متحمل و حساس بودند. نمونه‌های برگی، دو هفته پس از اعمال تنش شوری تهیه شدند. سپس استخراج پروتئین از بافت برگی صورت گرفت و الکتروفورز دوبعدی در گیاهان شاهد و تحت تیمارهای تنش شوری انجام شد. مقایسه نتایج تجزیه پروتئوم در سطوح تنش نشان داد که تعداد 15 لکه پروتئینی تکرارپذیر با تغییر بیان متفاوت بین دو رقم متحمل و حساس مشترک بوده و تعداد پنج لکه پروتئینی منحصر به هر رقم متحمل و حساس تحت تنش تغییر بیان معنی‌دار داشتند. لکه‌های پروتئینی با استفاده از طیف‌سنجی جرمی شناسایی شدند و نتایج نشان داد که پروتئین‌های مشترک شناسایی شده بیشتر در گروه‌های عملکردی شامل دفاع آنتی‌اکسیدانی و چرخه کالوین طبقه‌بندی شدند. در حالی که سایر پروتئین‌ها در هر رقم بیشتر در فعالیت آنتی-اکسیدانی نقش داشتند. در مجموع نتایج نشان داد که بین دو رقم مورد مطالعه از لحاظ پاسخ مورفو-فیزیولوژیکی به تنش شوری تفاوت معنی‌دار وجود دارد و رقم متحمل نیک‌نژاد پاسخ پروتئینی مناسبتری تحت تنش نشان می‌دهد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study morpho-physiological change and protein pattern of tolerant and susceptible wheat cultivars under salinity stress by using proteome analysis

نویسندگان [English]

  • Mohammad Reza Naghavi 1
  • Marouf Khalili 2
1 Assistant Professor, Department of Agriculture, Payame Noor University, Tehran, Iran.
2 Associate Professor, Department of Agriculture, Payame Noor University, Tehran, Iran
چکیده [English]

In order to investigate the mechanism of tolerance to salt stress in wheat, a factorial experiment basis of completely randomized design with four replications in greenhouse media were conducted. The factors of this experiment were included salinity of sodium chloride in four levels of zero (control), 100, 200 and 300 mM and two wheat cultivars namely Niknejhad and Pishtaz as a representative of tolerant and susceptible cultivars, respectively. Leaf samples were prepared two weeks after the starting of salt stress. Then, extraction of protein from leaf tissue was done and two-dimensional electrophoresis in control plants and under salt stress plants were carried out. The results of proteome analysis revealed that 15 replicated protein spots with different expression variations were common between two tolerant and susceptible cultivars, and five protein spots of unique to each tolerant and susceptible cultivar, had significantly expression variations under stress. Protein spots were detected by mass spectrometry and the results showed that more number from commonly detected proteins were classified in functional groups including antioxidant defense and Calvin cycle, while other proteins in each cultivar had more roles in antioxidant activity. Overall, the results showed that there was a significant difference between two cultivars in terms of morpho-physiological response to salt stress, and the Niknejhad cultivar showed a better protein response under stress.

کلیدواژه‌ها [English]

  • Proteome analysis
  • responsive proteins to stress
  • tolerance of salt stress
  • Wheat
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