Effect of Rhizoglomus irregulare symbiosis on reducing disease severity in tomato plants  infected with Tomato bushy stunt virus with emphasis on the expression of  methylation- related genes

Khoshkhati, Neda; Eini, Omid; KOOLIVAND, Davoud

doi:10.30473/cb.2020.54419.1818

Document Type : Research Paper

Authors

¹ Ph. D. Candidate, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

² Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

³ Assistant Professor, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

https://doi.org/10.30473/cb.2020.54419.1818

Abstract

Tomato bushy stunt virus (TBSV), a member of the genus Tombusvirus is one of the causal agents for curly disease in tomato plants. In this study, the interaction between a mycorrhizal fungus, Rhizoglomus irregular, and TBSV in tomato plants was investigated. In a completely randomized design experiment, tomato seedlings were inoculated with R. irregular and after four weeks they were inoculated with TBSV. Four treatments were included: control plants (C), TBSV -infected plants (V), mycorrhizal plants (M), TBSV -infected mycorrhizal plants (MV). Nineteen days after inoculation the infected plants were tested for symptom production and virus accumulation. Results of symptoms evaluation based on the disease severity index showed a lower disease severity in MV plants compared with V plants. Supporting this result, a lower level of virus accumulation was observed in V plants which was more significant at long-term infection. The expression of methylation-related genes including ADK, HEN1 and MET1 was tested by Real-time PCR. Results showed that the expression of these genes was significantly higher in MV plants as compared with V plants. An increase in the expression of methylation-related genes in MV plants indicates that resistance to the virus is likely to occur through methylation and also supports the lower level of virus accumulation in MV plants.

Keywords

20.1001.1.22520783.1399.10.30.4.8

Main Subjects

Plant Disease and Biotechnology

References

Agrios JN (2010) Plant pathology. Fifth Edition. Translated by Izadpanah K, Bani Hashemi Z, Rahimian H, Minasian V, Iage Publications.

Aseel DG, Rashad YM, Hammad SM (2019) Arbuscular mycorrhizal fungi trigger transcriptional expression of flavonoid and chlorogenic acid biosynthetic pathways genes in tomato against Tomato Mosaic Virus. Sci. Rep. Volume 9, Article number: 9692.

Arunachalam P, Radhakrishnan V, Mathew SK, Kumar PS (2002) Reaction of bitter gourd genotypes against distortion mosaic virus. Int. J. Veg. Sci. 29: 55-57.

Barbieri M, Acciarri N, Sabatini E, Sardo Accotto GP, Pecchioni N (2010) Introgression of resistance to two Mediterranean virus species causing Tomato Yellow Leaf Curl into a valuable traditional tomato variety. J. Plant Pathol. 92: 458-493.

Ebrahimi S, Eini, O, Koolivand D (2020) Arbuscular mycorrhizal symbiosis enhances virus accumulation and attenuates resistance-related gene expression in tomato plants infected with Beet curly top Iran virus. J. Plant Dis. Prot. 1-8.

Fakhro A, Andrade-Linares DR, Von BS, Bandte M, Büttner C, Grosch, R (2010). Impact of Piriformospora indica on tomato growth and on interaction with fungal and viral pathogens. Mycorrhiza., 20: 191-200.

Friedmann M, Lapidot M, Cohen S, Pilowsky M, (1998) A novel source of resistance to tomato yellow leaf curl virus exhibiting a symptomless reaction to viral infection. J. Am. Soc. Hortic. Sci. 123: 1004-1007.

Gerik JS, Duffus JE, Perry R, Stenger DC, Van Maren, AF (1990) Etiology of tomato plant decline in California desert. Phytopathol. 80: 1352-1356.

Gernns H, Alten H, Poehling HM (2001) Arbuscular mycorrhiza increased the activity of a biotrophic leaf pathogen–is a compensation possible? Mycorrhiza 11: 237-243.

Harris R, Ochoa-Corona R, Lebas B, Timudo O, Stewart F, Alexander B (2006) Broad detection and diagnosis of viruses of the genus Tombusvirus by RT-PCR coupled to single strand conformation polymorphism analysis. In: Proceedings of the 7th Australasian Plant Virology Workshop, Rottnest Island, Perth, Australia, November 8-11, p. 48.

Hildebrandt U, Regvar M, Bothe H (2007) Arbuscular mycorrhiza and heavy metal tolerance. Phytochemistry 68: 139-146.

Jafarpour B, Sabokkhiz MA (2010) Survey of Tomato yellow leaf curl virus (TYLCV) and Tomato bushy stunt virus (TBSV) in Razavi Korasan province. Petria. 20: p 153.

Khoshnazar F, Eini O (2016) Response of tomato cultivars to agroinfection with Beet curly top Iran virus. J. Crop Prot. 5: 473-482.

Law JA, Jacobsen SE (2010) Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat. Rev. Genet.11: 204-220.

Lecoq K, Belloc I, Desgranges C, Daignan-Fornier B (2001) Role of adenosine kinase in Saccharomyces cerevisiae: Identification of the ADO1 gene and study of the mutant phenotype. Yeast 18: 335-342.

Liu J, Maldonado‐Mendoza I, Lopez‐Meyer M, Cheung F, Town CD, Harrison MJ (2007) Arbuscular mycorrhizal symbiosis is accompanied by local and systemic alterations in gene expression and an increase in disease resistance in the shoots. The Plant. J. 50: 529-544.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. methods 25: 402-408.

Maffei G, Miozzi L, Fiorilli V, Novero M, Lanfranco L, Accotto G P (2014) The Arbuscular mycorrhizal symbiosis attenuates symptom severity and reduces virus concentration in tomato infected by Tomato yellow leaf curl Sardinia virus (TYLCSV). Mycorrhiza 24: 179-186.

Manners O, Baquero-Perez B, Whitehouse A (2019) m6A: Widespread regulatory control in virus replication. Bba-Gene Regul Mech. 1862(3): 370-381.

Martinez-Perez M, Aparicio F, Lopez-Gresa MP, Belles JM, Sanchez-Navarro JA, Pallas V (2017) Arabidopsis m(6)A demethylase activity modulates viral infection of a plant virus and the m(6)A abundance in its genomic RNAs. P Natl Acad Sci. 114: 10755-10760.

Matzke MA, Kanno T, Matzke AJM (2015) RNA-directed DNA methylation: the evolution of a complex epigenetic pathway in flowering plants. Annu. Rev. Plant Biol. 66: 243-267.

Miozzi L, Catoni M, Fiorilli V, Mullineaux PM, Accotto GP, Lanfranco L (2011) Arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long- term virus accumulation. Mol Plant Microbe In. 24: 1562-1572.

Nawaz H, Umer M, Bano S, Usmani A, Naseer, M (2014). Research review on tomato bushy stunt virus disease complex. J. Nat. Sci. Res. 4: 18-23.

Plotnikova A, Osipenko A, Masevic ius V, Vilkaitis G, Klimas auskas S (2014) Selective Covalent Labeling of miRNA and siRNA Duplexes Using HEN1 Methyltransferase. J. Am. Chem. Soc. 136: 13550-13553.

Pozo MJ, Azcón-Aguilar C (2007) Unraveling mycorrhiza-induced resistance. Current opinion in plant biology 10: 393-398.

Pozo MJ, Jung SC, López-Ráez JA, Azcón-Aguilar C (2010) Impact of Arbuscular mycorrhizal symbiosis on plant response to biotic stress: the role of plant defence mechanisms. In Arbuscular mycorrhizas: physiol. pp. 193-207.

Raja P, Sanville BC, Buchmann RC, Bisaro DM (2008) Viral Genome Methylation as an Epigenetic Defense against Geminiviruses. J. Virol. 82: 8997-9007.

Rodrıguez-Negrete E A, Lozano-Dura´ n R, Piedra-Aguilera A, Cruzado L, Bejarano E R, Castillo AG (2013) Geminivirus Rep protein interferes with the plant DNA methylation machinery and suppresses transcriptional gene silencing. New Phytol. 199: 464-475.

Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol, 132: 365-386.

Shaul O, Galili S, Volpin H, Ginzberg I, Elad Y, Chet I, Kapulnik Y (1999) Mycorrhiza-induced changes in disease severity and PR protein expression in tobacco leaves. Mol Plant Microbe In. 12: 1000-1007.

Sieverding E, Silva G, Berndt R, Oehl F. (2014) Rhizoglomus, a new genus of the Glomeraceae. Mycotaxon. 129: 373-386.

Timonen S, Smith SA, Smith SE (2001) Microtubules of the mycorrhizal fungus Glomus intraradices in symbiosis with tomato roots. Can. J. Bot. 79: 307-313.

Varga S, Soulsbury CD (2018) Arbuscular mycorrhizal fungi change host plant DNA methylation systemically. Plant Biol.

Wu B, Grigull, J, Ore M O, Morin S, White KA (2013) Global Organization of a Positive-strand RNA Virus Genome. PLOS Pathogens. 9: e1003363.

Wang H, Hao L, Shung CY, Sunter G, Bisaro D M (2003) Adenosine kinase isinactivated by geminivirus AL2 and L2 proteins. Plant Cell. 15: 3020-3032.

Wang J, Sun N, Deng T, Zhang L, Zuo K (2014) Genome-wide cloning, identification, classification and functional analysis of cotton heat shock transcription factors in cotton (Gossypium hirsutum). BMC Genomics. 15: 961.

Wehner J, Antunes P M, Powell JR, Mazukatow J, Rillig MC (2010) Plant pathogen protection by arbuscular mycorrhizas: a role for fungal diversity? Pedobiologia 53: 197-201.

Whipps JM, (2004) Prospects and limitations for mycorrhizas in biocontrol of root pathogens. Can. J. Bot. 82: 1198-1227.

Yamamura Y, Scholthof HB (2005) Tomato bushy stunt virus: a resilient model system to study virus-plant interactions. Mol. Plant Path. 6: 491-502.

Yang X, Xie Y, Raja P, Li S, Wolf JN, Shen Q, Zhou X (2011) Suppression of Methylation Mediated Transcriptional Gene Silencing by bC1-SAHH Protein Interaction during Geminivirus-Betasatellite Infection. PLoS Pathog. 7: e1002329.

Crop Biotechnology

Effect of Rhizoglomus irregulare symbiosis on reducing disease severity in tomato plants infected with Tomato bushy stunt virus with emphasis on the expression of methylation- related genes

References

References

Volume 10, Issue 30 - Serial Number 1
September 2020
Pages 43-55

Effect of Rhizoglomus irregulare symbiosis on reducing disease severity in tomato plants infected with Tomato bushy stunt virus with emphasis on the expression of methylation- related genes

References

References

Volume 10, Issue 30 - Serial Number 1September 2020Pages 43-55

Volume 10, Issue 30 - Serial Number 1
September 2020
Pages 43-55