Effect of Rhizoglomus irregulare symbiosis on reducing disease severity in tomato plants  infected with Tomato bushy stunt virus with emphasis on the expression of  methylation- related genes

Khoshkhati, Neda; Eini, Omid; KOOLIVAND, Davoud

doi:10.30473/cb.2020.54419.1818

Effect of Rhizoglomus irregulare symbiosis on reducing disease severity in tomato plants infected with Tomato bushy stunt virus with emphasis on the expression of methylation- related genes

Document Type : Research Paper

Authors

¹ Ph. D. Candidate, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

² Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

³ Assistant Professor, Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

10.30473/cb.2020.54419.1818

Abstract

Tomato bushy stunt virus (TBSV), a member of the genus Tombusvirus is one of the causal agents for curly disease in tomato plants. In this study, the interaction between a mycorrhizal fungus, Rhizoglomus irregular, and TBSV in tomato plants was investigated. In a completely randomized design experiment, tomato seedlings were inoculated with R. irregular and after four weeks they were inoculated with TBSV. Four treatments were included: control plants (C), TBSV -infected plants (V), mycorrhizal plants (M), TBSV -infected mycorrhizal plants (MV). Nineteen days after inoculation the infected plants were tested for symptom production and virus accumulation. Results of symptoms evaluation based on the disease severity index showed a lower disease severity in MV plants compared with V plants. Supporting this result, a lower level of virus accumulation was observed in V plants which was more significant at long-term infection. The expression of methylation-related genes including ADK, HEN1 and MET1 was tested by Real-time PCR. Results showed that the expression of these genes was significantly higher in MV plants as compared with V plants. An increase in the expression of methylation-related genes in MV plants indicates that resistance to the virus is likely to occur through methylation and also supports the lower level of virus accumulation in MV plants.

Keywords

20.1001.1.22520783.1399.10.30.4.8

Main Subjects

Plant Disease and Biotechnology

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