Payame Noor University Crop Biotechnology 2252-0783 13 2 2024 01 18 Bioinformatics study of reverse transcriptase sequence in viruses Bioinformatics study of reverse transcriptase sequence in viruses 45 52 10563 10.30473/cb.2023.69786.1933 FA Samira Karimi M.Sc. Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran. Mahin Pouresmaeil Post Doctoral Researcher, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili Journal Article 2023 09 21 Bioinformatics is an interdisciplinary science that utilizes information technologies to organize and analyze biological data. This science enables researchers to perform comprehensive and documented investigations on various biological problems without the need for expensive and time-consuming laboratory experiments .In this study, we acquired the reverse transcriptase (RT) sequence of eight virus strains from NCBI with the following accession numbers: NC_001497.2, NC_001648.1, NC_001839.2, NC_003977.2, AF053008.1, EF428979.1, NC_001802.1. We investigated the structural and functional characteristics, domains, and motifs. The analysis revealed that the proteins from the eight virus strains, belonging to different families, exhibited distinct properties that set them apart from one another. The analysis also showed that these proteins are found in the membrane, cytoplasm, and periplasm, and all of them contain at least one specific domain of the reverse transcriptase enzyme. Based on all the analyses performed, Cauliflower mosaic virus, Cassava vein mosaic virus, and Soybean chlorotic mottle virus, all belonging to the Caulimoviridae family, were suitable for producing RT enzymes. The ability of these viruses to adapt to different plant hosts could potentially lead to the development of more efficient and cost-effective methods for producing RT enzymes. This adaptability could also open up new possibilities for genetic engineering and biotechnology, enabling the development of more effective enzymes. Bioinformatics is an interdisciplinary science that utilizes information technologies to organize and analyze biological data. This science enables researchers to perform comprehensive and documented investigations on various biological problems without the need for expensive and time-consuming laboratory experiments .In this study, we acquired the reverse transcriptase (RT) sequence of eight virus strains from NCBI with the following accession numbers: NC_001497.2, NC_001648.1, NC_001839.2, NC_003977.2, AF053008.1, EF428979.1, NC_001802.1. We investigated the structural and functional characteristics, domains, and motifs. The analysis revealed that the proteins from the eight virus strains, belonging to different families, exhibited distinct properties that set them apart from one another. The analysis also showed that these proteins are found in the membrane, cytoplasm, and periplasm, and all of them contain at least one specific domain of the reverse transcriptase enzyme. Based on all the analyses performed, Cauliflower mosaic virus, Cassava vein mosaic virus, and Soybean chlorotic mottle virus, all belonging to the Caulimoviridae family, were suitable for producing RT enzymes. The ability of these viruses to adapt to different plant hosts could potentially lead to the development of more efficient and cost-effective methods for producing RT enzymes. This adaptability could also open up new possibilities for genetic engineering and biotechnology, enabling the development of more effective enzymes. https://cropbiotech.journals.pnu.ac.ir/article_10563_e49eb218f1ac8f819fa138109124aeaa.pdf