Payame Noor University Crop Biotechnology 2252-0783 15 2 2025 12 22 The Effect of Nano-Titanium Oxide and Methyl Jasmonate on the Total Phenolic and Flavonoid Contents in Cell Suspension Culture of Caper (Capparis spinosa) The Effect of Nano-Titanium Oxide and Methyl Jasmonate on the Total Phenolic and Flavonoid Contents in Cell Suspension Culture of Caper (Capparis spinosa) 13 25 12945 10.30473/cb.2026.74696.2009 FA Somayeh Taheri Department of Agriculture, Payeme Noor University, Tehran, Iran Nassim Zarinpanjeh Medicinal Plant Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Hamideh Khalaj Department of Agriculture, Payeme Noor University, Tehran, Iran Ghorban Shahriari Department of Agriculture, Payeme Noor University, Tehran, Iran Hadi Kalantari Khalilabad Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Journal Article 2025 05 25 This study was conducted to investigate callus production, cell suspension culture and the effect of induction on total phenolic content in Capparis spinosa. For this purpose, different explants (leaves and stems) were collected from adult plants and after disinfection, they were cultured under sterile conditions. The effect of different hormonal treatments including NAA and BAP compounds on callus induction was evaluated. The explants were cultured in MS medium containing different concentrations of these growth regulators. The results showed that the highest callus induction percentage (100%) was obtained in medium containing 1 mg/L 2,4-D and 0.5 mg/L BAP. The results of the variance analysis of data related to the amount of total phenol and flavonoids obtained from the cell suspension culture of the caper plant showed that the interaction effect of titanium dioxide nanoparticles and methyl jasmonate was significant at the 99% probability level. The highest level of total phenols (60.80 mg/g DW) and total flavonoids (49.36 mg/g DW) were related to the use of nano-titanium oxide 25 μg/L and methyl jasmonate 100 μmol as elicitor in the suspension culture medium. These findings can be used to optimize the production of bioactive compounds from C. spinosa under controlled in vitro conditions. This study was conducted to investigate callus production, cell suspension culture and the effect of induction on total phenolic content in Capparis spinosa. For this purpose, different explants (leaves and stems) were collected from adult plants and after disinfection, they were cultured under sterile conditions. The effect of different hormonal treatments including NAA and BAP compounds on callus induction was evaluated. The explants were cultured in MS medium containing different concentrations of these growth regulators. The results showed that the highest callus induction percentage (100%) was obtained in medium containing 1 mg/L 2,4-D and 0.5 mg/L BAP. The results of the variance analysis of data related to the amount of total phenol and flavonoids obtained from the cell suspension culture of the caper plant showed that the interaction effect of titanium dioxide nanoparticles and methyl jasmonate was significant at the 99% probability level. The highest level of total phenols (60.80 mg/g DW) and total flavonoids (49.36 mg/g DW) were related to the use of nano-titanium oxide 25 μg/L and methyl jasmonate 100 μmol as elicitor in the suspension culture medium. These findings can be used to optimize the production of bioactive compounds from C. spinosa under controlled in vitro conditions. https://cropbiotech.journals.pnu.ac.ir/article_12945_c9823c5001045223b32097bddf384e28.pdf