Payame Noor University Crop Biotechnology 2252-0783 10 2 2021 03 18 Identification and evaluation of relation between regulatory elements of the promoter region of DLV gene family and gene expression in Aeluropus littoralis Identification and evaluation of relation between regulatory elements of the promoter region of DLV gene family and gene expression in Aeluropus littoralis 95 109 7918 10.30473/cb.2021.58873.1836 FA Maryam Chalekaei Ph.D. Candidate of Agricultural Biotechnology, Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran. Ali Dehestani Assistant Professor of Molecular Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. Ali Reza Abbasi Associate Professor, Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran. Seyyed Hamidreza Hashemi-petroudi Assistant professor, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. 0000-0002-0870-1691 Journal Article 2020 12 30 In this study, the type and frequency of regulatory elements in the promoter regions of DVL gene family in Aeluropus littoralis were studied. Relative expression of AlDVL8 gene as a member of this family was also measured under the salinity stress, salicylic acid, gibberellic acid and cytokinin. The results of the promoter study indicated that, this gene family has different regulatory elements for responding to stresses and hormones. Some of these regulatory elements are present in the promoter region of all genes, possibly indicating the general role of DVLs. Some others are present only in the promoter region of some genes that may be related to their specific activity. Treatments other than cytokinin increased gene expression in the shoot at 3, 12, and 24 hours and decreased expression at 6 hours. Cytokinin treatment at all times increased gene expression. In the root, almost the reverse trend of gene expression was observed, so that at 6 hours, increased gene expression was observed in all treatments, and at 12 and 24 hours, decreased expression was observed in all treatments. The results of this study showed that the expression of AlDVL8 gene in shoot and root organs was induced by experimental treatments and its expression was inverse in these two different tissues. Due to the changes in hormones during stress, expression induction of this gene family, and the presence of stress-responsive elements in the promoter regions of these genes, this gene family can be suggested as a candidate for stress tolerance. In this study, the type and frequency of regulatory elements in the promoter regions of DVL gene family in Aeluropus littoralis were studied. Relative expression of AlDVL8 gene as a member of this family was also measured under the salinity stress, salicylic acid, gibberellic acid and cytokinin. The results of the promoter study indicated that, this gene family has different regulatory elements for responding to stresses and hormones. Some of these regulatory elements are present in the promoter region of all genes, possibly indicating the general role of DVLs. Some others are present only in the promoter region of some genes that may be related to their specific activity. Treatments other than cytokinin increased gene expression in the shoot at 3, 12, and 24 hours and decreased expression at 6 hours. Cytokinin treatment at all times increased gene expression. In the root, almost the reverse trend of gene expression was observed, so that at 6 hours, increased gene expression was observed in all treatments, and at 12 and 24 hours, decreased expression was observed in all treatments. The results of this study showed that the expression of AlDVL8 gene in shoot and root organs was induced by experimental treatments and its expression was inverse in these two different tissues. Due to the changes in hormones during stress, expression induction of this gene family, and the presence of stress-responsive elements in the promoter regions of these genes, this gene family can be suggested as a candidate for stress tolerance. https://cropbiotech.journals.pnu.ac.ir/article_7918_bd049eb6f518481160d3d4f7b8f8f73c.pdf