Increasing of Chavicol o-Methyl Transfrase Gene Expression and Catalase and Ascorbate Peroxidase Enzymes ctivity of Ocimum basilicum by Chitosan

Naderi, Saleh; Fakheri, Baratali; Esmailzadeh Bahabadi, Sedigheh

Increasing of Chavicol o-Methyl Transfrase Gene Expression and Catalase and Ascorbate Peroxidase Enzymes ctivity of Ocimum basilicum by Chitosan

Document Type : Research Paper

Authors

¹ Ph.D student of Plant Breeding, Zabol University, Zabol, Iran.

² Associate Professor, Department of Plant Breeding and Biotechnology, Zabol University, Zabol, Iran.

³ Assistant Professor, Department of Biology, Zabol University, Zabol, Iran.

Abstract

Basil (Ocimum basilicum L.), a medicinal plant of the Lamiaceae family, is used in traditional Iranian medicine. Essential oils of basil are composed of phenylpropanoids. Chavicol o-methyl and eugenol o-methyl are the most important components in essential oil of basil. Chitosan, main compound of fungal species could be used as biotic elicitor to improve secondary metabolites. In the present study, the effect of chitosan on methylchavicol content, chavicol o-methyl transfrase gene expression and enzymes activity of catalase, ascorbate peroxidase was evaluated. The plants were treated at pre flowering stage with 2 g/L chitosan and harvested after 1, 2, 3, and 5 days. Essential oils analysis showed that methylchavicol increased under chitosan compare to untreated plants. Chavicol o-methyl transfrose gene expression and antioxidant enzymes activity increased significantly after chitosan. Totally, changes in gene expression in different harvest stages are consistent with methylchavicol changes. Thus, chitosan increased methylchavicol by increasing CVOMT gene expression.

Keywords

20.1001.1.22520783.1393.4.6.1.5

Main Subjects

Biotic and Abiotic stress

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