Bioinformatics
Samira Karimi; Mahin Pouresmaeil
Abstract
Bioinformatics is an interdisciplinary science that utilizes information technologies to organize and analyze biological data. This science enables researchers to perform comprehensive and documented investigations on various biological problems without the need for expensive and time-consuming laboratory ...
Read More
Bioinformatics is an interdisciplinary science that utilizes information technologies to organize and analyze biological data. This science enables researchers to perform comprehensive and documented investigations on various biological problems without the need for expensive and time-consuming laboratory experiments .In this study, we acquired the reverse transcriptase (RT) sequence of eight virus strains from NCBI with the following accession numbers: NC_001497.2, NC_001648.1, NC_001839.2, NC_003977.2, AF053008.1, EF428979.1, NC_001802.1. We investigated the structural and functional characteristics, domains, and motifs. The analysis revealed that the proteins from the eight virus strains, belonging to different families, exhibited distinct properties that set them apart from one another. The analysis also showed that these proteins are found in the membrane, cytoplasm, and periplasm, and all of them contain at least one specific domain of the reverse transcriptase enzyme. Based on all the analyses performed, Cauliflower mosaic virus, Cassava vein mosaic virus, and Soybean chlorotic mottle virus, all belonging to the Caulimoviridae family, were suitable for producing RT enzymes. The ability of these viruses to adapt to different plant hosts could potentially lead to the development of more efficient and cost-effective methods for producing RT enzymes. This adaptability could also open up new possibilities for genetic engineering and biotechnology, enabling the development of more effective enzymes.
Bioinformatics
Mahin Pouresmaeil; Maghsoud Pazhouhandeh
Abstract
Today, the genome sequence of most organisms has been identified, and this information is useful in understanding the function and characteristics of organisms. In the meantime, there is unprocessed information that can be used to study unknown proteins and genes with the advancement of technology and ...
Read More
Today, the genome sequence of most organisms has been identified, and this information is useful in understanding the function and characteristics of organisms. In the meantime, there is unprocessed information that can be used to study unknown proteins and genes with the advancement of technology and the use of bioinformatics tools. In this research, the sequence of a gene with unknown function from Arabidopsis thaliana with accession number of X91953.1 in NCBI database was used to investigate and study its structure and possible function. This gene is related to chromosome number one in Arabidopsis thaliana and with 676 base pairs, it produces a protein with 150 amino acids and a molecular weight of approximately 15 kD. By using bioinformatics servers, the characteristics of both gene and protein sequences were investigated and it was found that it has 18 types of regulatory motifs, the functions of some of which are known, which can be related to the response to light and the activity of Cis elements for expression in the meristem. The analyzes showed that this protein has 38 motifs, three of them are conserved with high frequency. This protein has a signal peptide at its Nt and is leaked into the extracellular space. Therefore, its presence in the intercellular space is more likely than the nucleus and intracellular organelles. There is also a regulation site of a microRNA on its transcript and this microRNA is active in response to salinity and also in the embryo. This unknown protein has about 90% homology with another protein in Arabidopsis with accession number of UPF0540 (At1g62000), which can be used for further studies to identify the role of the desired protein. This protein is expressed in 10 different tissues, mainly in embryo and seed endosperm. Based on all the analyzes carried out, two functions of seed coat differentiation and the biosynthesis of secreted substances due to light can be predicted for this protein. In the continuation of this work, laboratory methods are recommended for testing the functions attributed to this gene.
