Biotic and Abiotic stress
Maryam Chalekaei; Ali Dehestani; Ali reza Abbasi; Seyyed Hamidreza Hashemi-petroudi
Abstract
In this study, the type and frequency of regulatory elements in the promoter regions of DVL gene family in Aeluropus littoralis were studied. Relative expression of AlDVL8 gene as a member of this family was also measured under the salinity stress, salicylic acid, gibberellic acid and cytokinin. The ...
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In this study, the type and frequency of regulatory elements in the promoter regions of DVL gene family in Aeluropus littoralis were studied. Relative expression of AlDVL8 gene as a member of this family was also measured under the salinity stress, salicylic acid, gibberellic acid and cytokinin. The results of the promoter study indicated that, this gene family has different regulatory elements for responding to stresses and hormones. Some of these regulatory elements are present in the promoter region of all genes, possibly indicating the general role of DVLs. Some others are present only in the promoter region of some genes that may be related to their specific activity. Treatments other than cytokinin increased gene expression in the shoot at 3, 12, and 24 hours and decreased expression at 6 hours. Cytokinin treatment at all times increased gene expression. In the root, almost the reverse trend of gene expression was observed, so that at 6 hours, increased gene expression was observed in all treatments, and at 12 and 24 hours, decreased expression was observed in all treatments. The results of this study showed that the expression of AlDVL8 gene in shoot and root organs was induced by experimental treatments and its expression was inverse in these two different tissues. Due to the changes in hormones during stress, expression induction of this gene family, and the presence of stress-responsive elements in the promoter regions of these genes, this gene family can be suggested as a candidate for stress tolerance.
Molecular Plant Breeding
Khosro Mafakheri; Mohammad Reza Bihamta; Ali Reza Abbasi
Volume 6, Issue 14 , August 2016, , Pages 43-60
Abstract
In the current investigation, 22 SSR primer pairs were used to identify molecular markers associated with morphological traits in cowpea (Vigna unguiculata L.) based association analysis, markers produced 186 locations in 32 cowpea genotypes. 22 SSR primers pair amplified 8.45 for each primer location ...
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In the current investigation, 22 SSR primer pairs were used to identify molecular markers associated with morphological traits in cowpea (Vigna unguiculata L.) based association analysis, markers produced 186 locations in 32 cowpea genotypes. 22 SSR primers pair amplified 8.45 for each primer location and the average polymorphism information content (PIC) ranged from 0.445 to 0.25 (Vm25) and 0.625 (Vm5) were varied, respectively. Cluster analysis based on Dice, Jaccard and Simple Maching similarity coefficients based on molecular traits revealed primarily two major groups. Stepwise regression analysis between molecular data as independent variables, and morphological data as dependent variables was performed to identify informative markers associated with the studied traits. SSR loci associated with economic and biological yield, in normal conditions and in drought stress condition were Vm70 and Vm33, and Vm3 and Vm26 respectively. In drought stress condition the maximum variation of number of days to 50% flowering, days to 50% maturity of pods and pod length (99.9%, respectively) was accounted by Vm22, Vm31 and Vm26 markers. While in the normal condition the maximum variation of traits sheath thickness, width of pod and days to 50% flowering (99.9%) was accounted by Vm14, Vm34 and Vm34 markers. In both normal and drought conditions most of the used SSR primers showed significant association with the studied traits, so we can use these markers along with morphological traits in breeding cowpea genotypes to identify drought tolerant and hardy cultivars suitable for the preparation and use mapping populations.
Genetic Engineering and Gene Transformation
ali reza abbasi; Nahid Raanaian; Fariba Aboei; U. SONNEWALD; L. M. VOLL
Volume 3, Issue 4 , September 2013, , Pages 43-52
Abstract
Under stress conditions Reactive Oxygens Species (ROS) are produced and accumulate in plant leaves as a results of the oxidation of important cellular components like proteins, chlorophylls and lipids. Vitamin E is the collective term for tocochromonals. This lipid-soluble substance ...
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Under stress conditions Reactive Oxygens Species (ROS) are produced and accumulate in plant leaves as a results of the oxidation of important cellular components like proteins, chlorophylls and lipids. Vitamin E is the collective term for tocochromonals. This lipid-soluble substance acompanied by carotenoids, glutathion and other antioxidants and has an important role in keeping the integrity of photosynthetic membranes. Tocopherols and tocoterienols are amphipathic molecules and are able to detoxify ROS and lipid peroxyl radicals in lipophilic environments. Hemogentisate phytyl transferase (HPT) is the key enzyme in the vitamin E biosynthesis pathway. To silence HPT and invesitgate its role in tocopherol production RNAi thechnique was used and the results obtained revealed that tocopherol content was decreased in derived lines.
Molecular Plant Breeding
Nahid Raanaian; Ali reza Abbasi; Hassan Zeynali-Khanghah
Volume 3, Issue 4 , September 2013, , Pages 149-156
Abstract
Exposing plants to both biotic and abiotic stresses could lead to increased reactive oxygen species and in turn could induce oxidative stress. In order to increase scavenging capacity of oxidative agents, the different plant antioxidant activities also are arising. Among those, vitamin E includes a group ...
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Exposing plants to both biotic and abiotic stresses could lead to increased reactive oxygen species and in turn could induce oxidative stress. In order to increase scavenging capacity of oxidative agents, the different plant antioxidant activities also are arising. Among those, vitamin E includes a group of Fat-soluble antioxidants, which their synthesis is limited to photosynthetic organisms including plants, alga and cyanobacteria. In this study, the coding region of Arabidopsis Tocopherol cyclase gene; which catalyzes 2,3-dimethyl-5-phytyl-1,4-benzoquinone (DMPBQ) into γ –tocopherol; was introduced into pBin vector containing the coding region of GFP protein. Then, the obtained construct was transformed into tobacco plant through agrobacterium-mediated method. Leaves of 2-3 weak old seedlings were selected as explant and direct regeneration was performed. In order to confirmation of transgenic plants, PCR by using specific primers was carried out. Due to the fact that the pBin vector contains the kanamycin resistant gene, seeds belonging to the T0 transgenic plants were planted on medium containing kanamycin and green seedling were selected as transgenic T1 plant. In order to evaluate the effects of transferred gens on physiological parameters, mature T1 transgenic plant as well as the wild type plants were subjected to drought stress and relative water content was measured.
Molecular Genetics and Genetic Engineering
Fatemeh Pirasteh Broujeni; Mohammad Reza Naghavi; Alireza Abbasi; Hasan Soltanloo; Mojtaba Ranjbar; Sara Raeisy
Volume 3, Issue 5 , February 2013, , Pages 23-31
Abstract
Artemisia species due to have different compounds of terpenoids are considered the most important medical plants. Artemisinin, a sesquiterpene with antimalarial and anticancer properties, and tritenpenes, squalene and β-amyrin, are important medicinal compounds which are produced by Artemisia species. ...
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Artemisia species due to have different compounds of terpenoids are considered the most important medical plants. Artemisinin, a sesquiterpene with antimalarial and anticancer properties, and tritenpenes, squalene and β-amyrin, are important medicinal compounds which are produced by Artemisia species. Since farnesyl diphosphate is the precursor of all tri- and sesquiterpenes, expression of farnesyl diphosphate synthase (FDS), squalene synthase (SQS) and β-amyrin synthase in three developmental stages are studied in seven Artemisia species native of Iran by real-time PCR. Furthermore, artemisinin content was determined by HPLC. Our results showed A. annua has maximum artemisinin content in budding stage and A. diffusa and A. spicigeria have minimum artemisinin content in vegetative stage. In this manner expression of FDS has no difference between the species and although its effective role in biosynthesis of artemisinin, it is not useful to manipulate for increase of artemisinin. Also lower expression of SQS means we will have higher artemisinin but the revers is not true. Also A. scoparia in flowering stage is the best source to access of squalene and β-amyrin.
Molecular Plant Breeding
M Ghaderi; A.R Abbasi; A.H Salmanian
Volume 2, Issue 2 , September 2012, , Pages 39-47
Abstract
Drought is by far the most important environmental stress in agriculture and many efforts have been made to improve crop productivity under water-limiting conditions.Minimizing the ‘yield gap’ and increasing yield stability under different stress conditions are of strategic importance in guaranteeing ...
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Drought is by far the most important environmental stress in agriculture and many efforts have been made to improve crop productivity under water-limiting conditions.Minimizing the ‘yield gap’ and increasing yield stability under different stress conditions are of strategic importance in guaranteeing food for the future. Expansin is a protein super family with 4 families in vascular plants. Expansions are cell wall proteins which mediate acidic cell wall loosening through breaking hydrogen bonds between cellulose and glycan matrix. In this study pBIEXPA1 construct was constructed. Arabidopsis plants were transformed by pBIEXPA1 which contained nptII gene and AtEXPA1 gene which was under the control of CaMV35s promoter. Transformation was done by floral dip which did not need any tissue culture and transformed plants were T1. Transformed seedlings were able to remain green on MS medium containing 50 mg/L kanamycin. Transgenic plants were confirmed by PCR. As expected two 753 and 1080bp band were seen in transgenic plants.
