Plant Disease and Biotechnology
M.R Safarnejad; M Basirat; M.A Ebrahimi; H Safarpour; S Nazari; B Mahmoudi; S Ataie Kachoie
Volume 2, Issue 3 , January 2013, , Pages 1-12
Abstract
Rhizomania is one of the most important Sugar beet diseases throughout the world. The disease is caused by beet necrotic yellow vein virus (BNYVV). The Polymyxa betae (Keskin) is the only natural transmitting agent of the disease between the plants. The fungus is an obligate parasite and could not be ...
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Rhizomania is one of the most important Sugar beet diseases throughout the world. The disease is caused by beet necrotic yellow vein virus (BNYVV). The Polymyxa betae (Keskin) is the only natural transmitting agent of the disease between the plants. The fungus is an obligate parasite and could not be cultured in the media, then detection of fungus is done normally by microscopic observation. In order to facilitate detection process, present study is done to develop specific antibodies against this fungus by applying recombinant protein. The Glutathione-S-transferase (GST) as a specific immunogenic protein is a critical enzyme expressed in zoospores, sporangia and resting spores and could be a good candidate to develop a serological test for P. betae. For this aim, the DNA region encoding fungal GST gene was isolated and cloned into pET28a bacterial expression vector. Large scale expression of the recombinant protein was performed in Escherichia coli (Migula). Purification was carried out by applying immobilized metal affinity chromatography under native conditions. The purified recombinant GST protein was used for immunization of rabbit. Purification of immunoglobulin was performed by affinity chromatography using protein A column. The purified antibodies were applied for efficient detection of infected plant in serological assays.
Atefeh Nasiri; Asghar Mirzaie asl; Mohsen Aghaeizadeh; Ali Deljou; Bagher Mahmoudi
Volume 1, Issue 1 , March 2012, , Pages 77-83
Abstract
Several sources of cytoplasmic male sterility (CMS) have been described in beets. Sugar beet cultivars are producing only using the source of Owen CMS. Introducing Owen CMS from cultivated sugar beet, have been important role in development of hybrid cultivars and changed breeding methods in sugar beet. ...
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Several sources of cytoplasmic male sterility (CMS) have been described in beets. Sugar beet cultivars are producing only using the source of Owen CMS. Introducing Owen CMS from cultivated sugar beet, have been important role in development of hybrid cultivars and changed breeding methods in sugar beet. Sugar beet cultivars carring CMS Owen are semi fertile. In order to investigating cytoplasm variation and cytoplasmic male sterility source between cultivars, mitochondrial minisatellite and PCR-RFLP markers were used. Banding profiles of four cultivars and two male strile and fertile lines demonstrated the presence of Owen CMS in breeding process of these cultivars. Cytoplasmic variation was not observed between cultivars, but some progeny of cross between cultivar plants and a pollinator were sterile. It seems, other resource, besides Owen CMS have been used in producing some hybrid cultivars.
