Molecular Plant Breeding
Ashkboos Amini; Sheida Khadam kheir; Iraj Bernousi
Abstract
In order to evaluate variation and reaction of 40 wheat genotypes to salinity tolerance and assess the association of 27 microsatellite markers with salinity tolerance and morpho-physiological traits measured under normal and salt stress conditions, the present study was conducted. The results of analysis ...
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In order to evaluate variation and reaction of 40 wheat genotypes to salinity tolerance and assess the association of 27 microsatellite markers with salinity tolerance and morpho-physiological traits measured under normal and salt stress conditions, the present study was conducted. The results of analysis of variance in two environments showed significant difference among genotypes for all traits, indicating genetic variation among them. Based on the results of combined analysis of variance, the effect of the environment for all traits and the effect of Genotype × environment on most of the traits was significant and it was determined that the response of the evaluated genotypes to salinity stress was different In two environment, the highest variation was belonged to grain yield and its component and biological yield. Cluster analysis in salinity stress based on traits with significant genotype × environment, classified the genotypes into 3 groups i.e. tolerant moderate and sensitive. Association analysis using 27 microsatellite markers, SSR markers with 11 traits measured in two normal conditions and salinity stress for all genotypes was conducted through a mixed linear model (MLM) The study of population structure as a precondition for communication analysis showed that there are 2 probable subgroups (K = 2) in the population studied, which was confirmed by the results of the plot formula. The decomposition of the association analysis using 27 SSR markers with 11 traits measured in two conditions based on mixed linear model (MLM) was carried out using the population structure matrix. Based on the results, 29 locations had a significant relationship with the evaluated traits in the normal environment, while in the salinity stress environment, this number increased to 40 locations. The existence of common markers among some of the studied traits such as the significant associate between S16-1 and 3 traits in normal conditions and with 5 traits in the salinity medium can be due to the polytrophic effects of these markers and possibly the connectivity of genomic locations controlling these traits. S6-3 and S12-1 markers were identified as markers that associated with grain yield in salinity condition and S11-4 marker was identified as marker that related with biological yield in both environmental Conditions. Finally, given the results obtained, if the results are confirmed in other genetic fields, these markers can be used in corrective programs.
Bioinformatics
Hajar Nasrollahi; Farshid Talat; Iraj Bernousi; Mehdi Badri Anarjan
Volume 7, Issue 20 , March 2018, , Pages 1-12
Abstract
Diploid genome contains 8 genomes designated as A, B, C, D, E, F, G and K which have been identified in the genus Gossypium. The genome of A is limited only in two species as G. herbaceum and G.arboreum, and it is transferred from G. herbaceum to other species. The chloroplast genome (CP) of G. herbaceum ...
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Diploid genome contains 8 genomes designated as A, B, C, D, E, F, G and K which have been identified in the genus Gossypium. The genome of A is limited only in two species as G. herbaceum and G.arboreum, and it is transferred from G. herbaceum to other species. The chloroplast genome (CP) of G. herbaceum has 160,140 bp lengths with protected quadripartite structure. Single copy regions of chloroplast genome are separated by two inverted repeat regions with a large copy region with 88,709 bp also the single copy region and each small inverted repeat regions have 20,221 and 25,605bp. The plastidic' s genome has 113 single copy genes and 19 duplicated copy genes. Single copy genes are encoding of 79 genes for protein production, four ribosomal RNA genes and 31 transfer RNA genes. Result showed that among all plastid genes only 18 genes appeared to have 1-2 intron/s and when compared with chloroplast genome of two allotetraploid species. Ycf15 gene as the only duplicated gene, rpl22 was in G. herbaceum and in the two species has studied G. herbaceum, G. barbadense. But ycf15 gene in G. barbadense and both ycf15 and rpl22 genes were lost in G. barbadense and G. hirsutum. Though the high level of SSR protection in the chloroplast genome. SSRs are useful for genetic variation analysis since they have high efficiency against genomic SSRs.
