Genetic Engineering and Gene Transformation
S Shahbazi; N Safaie; A Mousavi; F Sanjarian; A Alizadeh
Volume 2, Issue 2 , September 2012, , Pages 73-85
Abstract
Fusarium Head Blight (FHB), which is caused commonly by Fusarium graminearum, has ability to result significant reduce in yield and also cause indirect losses due to the accumulation of potent mycotoxins (trichothecenes) in harvested grain as secondary metabolites; which are hazardous for human and animal. ...
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Fusarium Head Blight (FHB), which is caused commonly by Fusarium graminearum, has ability to result significant reduce in yield and also cause indirect losses due to the accumulation of potent mycotoxins (trichothecenes) in harvested grain as secondary metabolites; which are hazardous for human and animal. Trichothecene mycotoxins (such as DON) are potent protein synthesis inhibitors for eukaryotic organisms. Trans expression of AYT1 gene from S. cerevisiae capable of trichothecene 3-O-acetylation and converts DON to a less toxic acetylated form. One of the detoxification and resistance to mycotoxins is acetylation. The main goal of this study was to evaluate transgenic tobacco model plants to deoxynivalenol (DON). In order to detect expression of the AYT1 transgene, we added cMyc tag via PCR-Tagging method and introduced it into the model tobacco plants through Agrobacterium-mediated transformation in an attempt to detoxify DON. After confirmation of integrations of AYT1-cMyc into the tobacco genome by molecular analyses, Immuno-blotting and serological protein studies and trichothecene acetyl transferase activity analyses confirmed the expression of AYT1 and tolerance to 10 ppm concentration of DON in transgenic lines was observed.
