Proteomics
Samira Mardani Zonouz; Mahmoud Toorchi; Shaghayegh aslzad
Abstract
Drought is one of the most important factors causing abiotic stress in plants. Wheat, as a vital crop, is extensively cultivated in regions that face water scarcity at least during one period of the year. Proteomic approach is one of the ways to identify proteins involved in plant tolerance to water ...
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Drought is one of the most important factors causing abiotic stress in plants. Wheat, as a vital crop, is extensively cultivated in regions that face water scarcity at least during one period of the year. Proteomic approach is one of the ways to identify proteins involved in plant tolerance to water stress. In order to investigate the effect of water deficit stress on the root proteome pattern of desert-tolerant wheat, an experiment was conducted in the form of a completely randomized design with seven replications. Root proteins were extracted by TCA/acetone method and the protein expression pattern was analyzed using two-dimensional electrophoresis. Potential proteins involved in the response to water deficiency were identified by comparing the protein expression patterns under water deficit stress with the expression pattern in control conditions. The results revealed significant differences in root weight and root length at a 5% probability level, indicating the detrimental effects of water stress on plant roots. The proteomic analysis identified 98 reproducible protein spots, of which 10 exhibited statistically significant changes, with eight spots showing increased expression and one showing decreased expression. These protein spots were identified based on their molecular weight (MW) and isoelectric point (pI) through database searches. The identified proteins were classified into various functional categories related to stress response, including protein synthesis and accumulation, oxidative stress, response and defense against stress and metabolic pathways.
Bioinformatics
Seyyed Hamidreza Hashemi-petroudi; Samira Mohammadi; Esmaeil Bakhshandeh; Markus Kuhlmann
Abstract
From prokaryotes to higher eukaryotes, protein phosphatase 2Cs (PP2Cs) play a critical role in the stress response. For the purpose of identifying the AlPP2C gene and examining its expression, Aeluropus littoralis, a salt-secreting halophytic grass belonging to the Poaceae family, was genome-wildly analyzed. ...
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From prokaryotes to higher eukaryotes, protein phosphatase 2Cs (PP2Cs) play a critical role in the stress response. For the purpose of identifying the AlPP2C gene and examining its expression, Aeluropus littoralis, a salt-secreting halophytic grass belonging to the Poaceae family, was genome-wildly analyzed. Based on the unique structure of the PP2C domain, 34 AlPP2C genes were discovered and classified into ten evolutionary branches based on homology with Arabidopsis thaliana. According to exon-intron structural analyses, they possessed a wide range of exon counts. AlPP2Cs shared similar motif organization in the same evolutionary branches based on motif distribution. The motifs ABRE, MBS, DRE, STRE, and LTR, which are related with stress, were discovered in the promoter region of the AlPP2C. AlPP2Cs displayed varied expression patterns in leaf and root tissues in response to salt stress and recovery conditions, according to transcriptome analyses. The AlPP2C4 gene is only expressed in the root tissues. These results expand our understanding of the PP2C gene family and provide valuable information for future research on PP2Cs molecular function and biological processes studies.
