Tissue culture and Micropropagation
Zeinab Chaghakaboodi; Mahdi Kakaei; Alireza Zebarjadi; danial kahrizi
Abstract
Rapeseed (Brassica napus) is recognized as one of the most important oilseed crops worldwide and its development of cultivation has received attention due to the importance of importing oil. The current study aimed to investigate the effect of drought stress on some Rapeseed genotypes under tissue culture ...
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Rapeseed (Brassica napus) is recognized as one of the most important oilseed crops worldwide and its development of cultivation has received attention due to the importance of importing oil. The current study aimed to investigate the effect of drought stress on some Rapeseed genotypes under tissue culture and field conditions and to identify stable genotypes in the field. The possible responses of 14 different canola genotypes to Callus induction resulting from Hypocotyl cultivation and evaluation their drought tolerance were studied using Polyethylene Glycol 6000 (PEG 6000) at five different levels, including zero (as control), 10%, 20%, 30%, and 40% PEG concentrations based on a completely randomized design (CRD) with three replications. Measured traits included relative growth rate, growth rate, relative water content, and proline content of the Callus. Furthermore, in the field sector, the genotypes were investigated in four environments (two consecutive years in 2016-2018 under rainfed and irrigated conditions) based on randomized complete block design with three replications. According to the Callus culture results, the assessed traits, except the Proline content of Callus, decreased with increasing stress level. In laboratory conditions, genotype number seven (Dante) was introduced as the superior genotype. The results of Additive Main effects and Multiplicative Interaction (AMMI) analysis showed the significance of both additive effects of genotype and environment and the multiplicative effect of genotype × environment interaction. The results of cumulative additive effects (decomposition of variance) and multiplicative interaction effects (decomposition into principal components) showed that the first two components explained 53.02 and 33.65% of the variance of the interaction effect for oil yield. Dante and SLM-046 genotypes were introduced as stable genotypes.
Tissue culture and Micropropagation
Keyghobad Kaikavoosi; Altaf Hosseini Nadaf; Gholamreza Bakhshi khaniki
Volume 5, Issue 9 , June 2015, , Pages 29-38
Abstract
In most transformation studies it has been indicated that adding proline to the tissue culture medium can increase the callus induction frequency and reduce induction time. Adding proline to callus induction medium in this phase can affect the production of aromatic compounds in rice and if the goal ...
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In most transformation studies it has been indicated that adding proline to the tissue culture medium can increase the callus induction frequency and reduce induction time. Adding proline to callus induction medium in this phase can affect the production of aromatic compounds in rice and if the goal of exogenous gene transformation is increasing the rice aromatic associated secondary metabolites such as 2-acetyl-1-pyrroline (2AP), this amino acid can slipup the final results. Although, absence of amino acids such as proline can reduce callus induction percentage. In this research, callus induction from two indica rice varieties; Ambemohar 157 and Indrayani were optimized, using MS medium having various concentrations of 2,4-D, without using proline. The results revealed that 2.5 mgl-1 of 2,4-D for Ambemohar 157 and 4 mgl-1 for Indrayani can lead to better callus induction. These results indicated that absence of proline can be disregard by increasing of 2,4-D concentrations. Calluses obtained from the best hormone treatment were cultured on MS fortified with 0.01 mgl-1 NAA + (1, 2, 3, 4, 5) mgl-1 BAP for shoot regeneration. The highest percentage of regeneration was achieved on MS supplemented with 2 mgl-1and 3 mgl-1 for Ambemohar157 and Indrayani cultivar respectively. Proline contents in calli which were growth in MS medium supplemented with 500 mg/L of proline showed approximately 12 to 14 fold increase over the calli growth in non-proline added medium.
