Investigating the expression of nitrate transporter genes in response to nitrogen deficiency level in Arabidopsis

Saidi, Abbas; Hajibarat, Zohreh; Ghaffari, Mohammar Reza; Zeinalabedini, Mehrshad

doi:10.30473/cb.2023.67299.1904

Investigating the expression of nitrate transporter genes in response to nitrogen deficiency level in Arabidopsis

Document Type : Research Paper

Authors

¹ Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

² Agricultural Biotechnology Research Institute of Iran, Department of Systems and Synthetic Biology, Karaj, Iran. Agricultural Research, Education and Extension Organization (AREEO), Iran

10.30473/cb.2023.67299.1904

Abstract

Nitrogen is one of the most important components of biomolecules, amino acids, nucleotides, proteins, chlorophyll, and many plant hormones, which are essential and necessary for plant growth and development. In the condition of nitrogen deficiency very different responses such as yield reduction, leaf chlorosis, plant growth and root structure formation appears in phenotypes of plants. In the last decade, to increase the amount of biomass and as a result the yield of plants, a wide use of nitrogen has attracted the attention of researchers. In this study, the expression analysis of seven nitrate transporter genes (NRT2) was investigated in Arabidopsis in response to nitrogen deficiency stress at 4 and 7 days after this stress. The expression analysis of NRT2.3 and NRT2.4 genes showed increased expression at 7 days after applying nitrogen deficiency stress. But all the genes did not show a significant increase in expression at 4 days after N stress application. NRT2.4 gene showed a significant increase in 4 and 7 days after applying nitrogen stress compared to other genes. Overall, our results showed that increased nitrate transporter gene expression in leaves contributes to nitrogen uptake for plant growth and nitrogen accumulation in response to long-term low nitrogen stress. These findings can lead to a better understanding of the mechanism of low nitrogen tolerance and therefore the increase of other cultivars with nitrogen deficiency stresses.

Keywords

Main Subjects

Bioinformatics

References

Alboresi, A., Gestin, C., Leydecker, M. T., Bedu, M., Meyer, C., & Truong, H. N. (2005). Nitrate, a signal relieving seed dormancy in Arabidopsis. Plant, Cell & Environment, 28(4), 500-512. Fandika, I. R., Kemp, P. D., Millner, J. P., Horne, D., & Roskruge, N. (2016). Irrigation and nitrogen effects on tuber yield and water use efficiency of heritage and modern potato cultivars. Agricultural Water Management, 170, 148-157. Filleur, S., & Daniel-Vedele, F. (1999). Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display. Planta, 207, 461-469. Filleur, S., Dorbe, M. F., Cerezo, M., Orsel, M., Granier, F., Gojon, A., & Daniel-Vedele, F. (2001). An Arabidopsis T‐DNA mutant affected in Nrt2 genes is impaired in nitrate uptake. FEBS Letters, 489(2-3), 220-224. Garnett, T., Conn, V., Plett, D., Conn, S., Zanghellini, J., Mackenzie, N., ... & Kaiser, B. N. (2013). The response of the maize nitrate transport system to nitrogen demand and supply across the lifecycle. New Phytologist, 198(1), 82-94. Glass, A. D. (2003). Nitrogen use efficiency of crop plants: physiological constraints upon nitrogen absorption. Critical Reviews in Plant Sciences, 22(5), 453-470. Kiba, T., Feria-Bourrellier, A. B., Lafouge, F., Lezhneva, L., Boutet-Mercey, S., Orsel, M., ... & Krapp, A. (2012). The Arabidopsis nitrate transporter NRT2. 4 plays a double role in roots and shoots of nitrogen-starved plants. The Plant Cell, 24(1), 245-258. Krapp, A., David, L. C., Chardin, C., Girin, T., Marmagne, A., Leprince, A. S., ... & Daniel-Vedele, F. (2014). Nitrate transport and signalling in Arabidopsis. Journal of Experimental Botany, 65(3), 789-798. Lezhneva, L., Kiba, T., Feria‐Bourrellier, A. B., Lafouge, F., Boutet‐Mercey, S., Zoufan, P., ... & Krapp, A. (2014). The Arabidopsis nitrate transporter NRT 2.5 plays a role in nitrate acquisition and remobilization in nitrogen‐starved plants. The Plant Journal, 80(2), 230-241. Li, W., Wang, Y., Okamoto, M., Crawford, N. M., Siddiqi, M. Y., & Glass, A. D. (2007). Dissection of the AtNRT2. 1: AtNRT2. 2 inducible high-affinity nitrate transporter gene cluster. Plant Physiology, 143(1), 425-433. Ma, J. F., Yamaji, N., Mitani, N., Tamai, K., Konishi, S., Fujiwara, T., ... & Yano, M. (2007). An efflux transporter of silicon in rice. Nature, 448(7150), 209-212. Miller, A. J., Fan, X., Orsel, M., Smith, S. J., & Wells, D. M. (2007). Nitrate transport and signaling. Journal of Experimental Botany, 58(9), 2297-2306. Orsel, M., Krapp, A., & Daniel-Vedele, F. (2002). Analysis of the NRT2 nitrate transporter family in Arabidopsis. Structure and gene expression. Plant physiology, 129(2), 886-896. Rens, L. R., Zotarelli, L., Rowland, D. L., & Morgan, K. T. (2018). Optimizing nitrogen fertilizer rates and time of application for potatoes under seepage irrigation. Field Crops Research, 215, 49-58. Safi, A., Medici, A., Szponarski, W., Martin, F., Clément-Vidal, A., Marshall-Colon, A., ... & Krouk, G. (2021). GARP transcription factors repress Arabidopsis nitrogen starvation response via ROS-dependent and-independent pathways. Journal of Experimental Botany, 72(10), 3881-3901. Saidi, A., Hajibarat, Z., & Hajibarat, Z. (2020). Transcriptome analysis of Phytophthora infestans and Colletotrichum coccodes in tomato to reveal resistance mechanisms. Asia-Pacific J. Mol. Biol. Biotechnol, 28, 39-51. Tong, J., Walk, T. C., Han, P., Chen, L., Shen, X., Li, Y., ... & Qin, L. (2020). Genome-wide identification and analysis of high-affinity nitrate transporter 2 (NRT2) family genes in rapeseed (Brassica napus L.) and their responses to various stresses. BMC Plant Biology, 20, 1-16. Vidal, E. A., Alvarez, J. M., Araus, V., Riveras, E., Brooks, M. D., Krouk, G., ... & Gutiérrez, R. A. (2020). Nitrate in 2020: thirty years from transport to signaling networks. The Plant Cell, 32(7), 2094-2119. Von Wittgenstein, N. J., Le, C. H., Hawkins, B. J., & Ehlting, J. (2014). Evolutionary classification of ammonium, nitrate, and peptide transporters in land plants. BMC Evolutionary Biology, 14(1), 1-17. Wang, R., Xing, X., & Crawford, N. (2007). Nitrite acts as a transcriptome signal at micromolar concentrations in Arabidopsis roots. Plant Physiology, 145(4), 1735-1745. Wang, W., Hu, B., Yuan, D., Liu, Y., Che, R., Hu, Y., ... & Chu, C. (2018). Expression of the nitrate transporter gene OsNRT1. 1A/OsNPF6. 3 confers high yield and early maturation in rice. The Plant Cell, 30(3), 638-651. Yan, M., Fan, X., Feng, H., Miller, A. J., Shen, Q., & Xu, G. (2011). Rice OsNAR2. 1 interacts with OsNRT2. 1, OsNRT2. 2 and OsNRT2. 3a nitrate transporters to provide uptake over high and low concentration ranges. Plant, Cell & Environment, 34(8), 1360-1372. Zhang, T. Q., Chen, Y., & Wang, J. W. (2021). A single-cell analysis of the Arabidopsis vegetative shoot apex. Developmental Cell, 56(7), 1056-1074.