Investigating the growth and phytochemical characteristics in the in vitro culture of Nitraria schoberi under salt stress

Document Type : Research Paper

Authors

1 ., Department of Agriculture, Pyamen Noor University, Tehran, Iran

2 Medicinal Plants Research Center, ‎Institute of medicinal plants, ACECR, ‎Karaj, Iran.

3 Department of Agriculture, Pyamen Noor University, Tehran, Iran

4 , Medicinal plants Research Center, Institute of medicinal plants, ACECR, Karaj, Iran

5 PhD Student, Plant Bank, Iranian Biological Resource Center (IBRC), ACECR, Karaj, Iran

Abstract

To investigate the effect of salt stress on the growth and phytochemical characteristics in the in vitro cultivation of Nitraria schoberi, an experiment was conducted in the form of a factorial design with a completely random basis. The treatments included different concentrations of sodium chloride (0, 50 and 100 mM) and sodium sulfate (0, 10 and 20 mM). First, the seeds were germinated in vitro and then the cotyledon leaves with the hypocotyl of germinated seeds were placed as explants in MS cultivation environment with 2 mg/L BAP, 0.5 mg/L IBA, and the mentioned treatments. The explants were placed in the growth room under the conditions of 16 hours of light and 8 hours of darkness at a temperature of 25±2. After 4 weeks, stem length, number of leaves, and total phenol and flavonoids were measured. The results of variance analysis showed that different levels of salt have a significant effect (at the 99% level) on the growth indices of seedlings such as the length of the stem and the number of leaves, as well as on the total amount of phenol and flavonoid in the tissue of leaves. Based on comparisons, the maximum length of the stem (4.3 cm) and the number of leaves (5.5) related to the cultivation environment without salt and the highest amount of total phenol (35.37 mg/g) and flavonoid (24.6 mg/g) g/g) related to the cultivation environment containing 50 mM sodium chloride with 10 mM sodium sulfate.

Keywords

Main Subjects

References

Ahmed Ali, A.M., Mohamed El-Nour, M.E., Mohamed Yagi, S. (2018). Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors. Journal of Genetic Engineering and Biotechnology.16 (2), 677-682. Ampofo, JO., & Ngadi, M. (2021). Stimulation of the phenylpropanoid pathway and antioxidant capacities by biotic and abiotic elicitation strategies in common bean (Phaseolus vulgaris) sprouts. Process Biochem., 100, 98-106. Angelova, Z., Georgiev, S., Roos, W. (2006). Elicitation of plants. Biotechnology & Biotechnological Equipment., 20, 72-83. Behdad, A., Mohsenzadeh, S., Azizi, M. and Moshtaghi, N. (2020). Salinity effects on physiological and phytochemical characteristics and gene expression of two Glycyrrhiza glabra L. populations. phytochemistry, 171, 112236 Boyer, J.S. (1982). Plant productivity and environment. Science, 218, 443-448. Falcinelli, B., Sileoni, V., Marconi, O., Perretti, G., Quinet, M., Lutts, S., Benincasa, P. (2017). Germination under Moderate Salinity Increases Phenolic Content and Antioxidant Activity in Rapeseed (Brassica napus var oleifera Del.) Sprouts. Molecules, 22, 1377. Flowers, T. J., Colmer, T.D. (2008). Salinity tolerance in halophytes. New Phytologist, 179(4), 945-963. Gill, S.S., Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48(12), 909-930. Hasanuzzaman, M., Nahar K, Fujita M. (2012). Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In Ecophysiology and Responses of Plants under Salt Stress; Springer, New York, NY, USA, pp. 25–87. Hasanuzzaman, M., Nahar, K., Fujita, M. (2012). Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In Ecophysiology and Responses of Plants under Salt Stress; Springer, New York, NY, USA, pp. 25–87. Hawrylak-Nowak, B., Dresler, Stasi., nska-Jakubas, M., Wójciak, M., Sowa, I., Matraszek-Gawron, R. (2012). NaCl-Induced Elicitation Alters Physiology and Increases Accumulation of Phenolic Compounds in Melissa officinalis L. J. Mol. Sci., 22, 6844. Iraji Mareshk, M. and Moghaddam M. (2021). Physiological and biochemical responses of Mexican marigold (Tagetes minuta L.) to mycorrhizal fungi application under salinity stress condition. Journal of Iranian Plant Ecophysiological Research, 60, 79-94. Jogaiah, S., Govind, S.R., Tran, L.S. (2013). Systems biology-based approaches toward understanding drought tolerance in food crops. Critical Reviews in Biotechnology, 33(1), 23-39. Keisham, M., Mukherjee, S., Bhatla, SC.(2018). Mechanisms of sodium transport in plants- progresses and challenges. Int. J. Mol. Sci. 19, 647. Khan, M.I., Nazir, F., Asgher, M., Per, T. S., Khan, N.A. (2015). Selenium and sulfur influence ethylene formation and alleviate cadmium-induced oxidative stress by improving proline and glutathione production in wheat. Journal of Plant Physiology, 173,9-18. Kheirabadi, S., Zarinpanjeh, N., Ebrahimi, M.A., Bakhshi Khaniki, G.R., Naseri, H. R. (2020). Effective in vitro seed germination and direct regeneration from cotyledonary leaf explants of Nitraria schoberi. Iranian Journal of Genetics and Plant Breeding, 9 (1), 10-16. Kim, J.Y., Lee, S. H., Lee, J. S. (2012). Differential responses of three perennial grasses to salt stress by sodium chloride, Growth, ion accumulation, the role of anti-oxidant enzymes and osmolyte concentration. African Journal of Biotechnology, 11(40), 9719-9729. Naseri, H.R., Jafari, M., Sadeghi Sangdehi, S.A., Mohammadzadeh Khani, H., Saffariha, M. (2011). The effect of salinity on seed germination and the growth of Qara Dagh species (Nitraria schoberi). Journal of Rangland, 5(1), 81-90. (In Persian). Munns, R., Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681. Murashige, T., Skoog, F. (1962). A Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant, 15, 473-497. Negrao, S., Schmockel, SM., Tester, M.(2017). Evaluating physiological responses of plants to salinity stress. Ann. Bot., 119, 1-11. Pangin, A., Lartseva, L., Loskutnikova, V., Shakhov, V., Popova, E., Skrypnik, L., Krol, O. (2023). Effect of Salinity Stress on Phenolic Compounds and Antioxidant Activity in Halophytes Spergularia marina (L.) Griseb. and Glaux maritima L. Cultured In Vitro. Plants, 12, 1905. Patel, H., & Krishnamurthy, R. (2013). Elicitors in plant tissue culture. Journal of Pharmacognosy and Phytochemistry, 2, 60-65. Rafiee, D., Ebrahimi, MA., Qavami, N., Zarinpanjeh, N. (2022). The Effect of NaCl and Salicylic Acid on Total Phenolic and Flavonoid Contents in Suspension Culture of Nitraria schoberi. Journal of Medicinal Plants and By-products. Doi, 10.22092/JMPB.2022.358302.1465 Rai, M. K., Kalia, R. K., Singh, R., Gangola, M. P. and Dhawan, A. K. (2011). Developing stress tolerant plants through in vitro selection- an overview of the recent progress. Environmental and Experimental. Botany, 71, 89-98. Rathore, S., Singh, N., Singh, SK. (2014). Influence of NaCl on biochemical parameters of two cultivars of Stevia rebaudiana regenerated in vitro. J Stress Physiol Biochem., 10, 287-29. Rouphael, Y., Petropoulos, SA., Cardarelli, M., Colla, G. (2018). Salinity as eustressor for enhancing quality of vegetables. Sci. Hortic., 234, 361-369. Samec, D., Lini´c, I., Salopek-Sondi, B. (2021). Salinity Stress as an Elicitor for Phytochemicals and Minerals Accumulation in Selected Leafy Vegetables of Brassicaceae. Agronomy, 11, 361. Sharifi-Rad, J., Hoseini-Alfatemi, SM., Sharifi-Rad, M., Iriti, M. (2014). Free radical scavenging and antioxidant activities of different parts of Nitraria schoberi L. J Biol Act Prod Nat., 4(1), 44-51. Younessi-Hamzekhanlu, M., Dibazarnia, Z., Oustan, S., Vinson, T., Katam, R., Mahna, N. (2021). Mild Salinity Stimulates Biochemical Activities and Metabolites Associated with Anticancer Activities in Black Horehound (Ballota nigra L.). Agronomy, 11,2538. Zhao, J., Davis, L.C., Verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv, 23, 283- 333. Zheleznichenko, T., Banaev, E., Asbaganov, S., Voronkova, M., Kukushkina, T., Filippova, E., Mazurkova, N., Shishkina, L., Novikova, T. (2018). Nitraria schoberi L. hairy root culture as a source of compounds with antiviral activity against influenza virus subtypes А (H5N1) and А (H3N2). 3 Biotech., 8, 260.

Files

Share

How to cite

Statistics