Analysis of root proteome in wheat (Kavir variety) under drought stress

Document Type : Research Paper

Authors

1 M.Sc. in Agricultural Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, IRAN

3 M.Sc. in Agricultural Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

Abstract

Drought is one of the most important factors causing abiotic stress in plants. Wheat, as a vital crop, is extensively cultivated in regions that face water scarcity at least during one period of the year. Proteomic approach is one of the ways to identify proteins involved in plant tolerance to water stress. In order to investigate the effect of water deficit stress on the root proteome pattern of desert-tolerant wheat, an experiment was conducted in the form of a completely randomized design with seven replications. Root proteins were extracted by TCA/acetone method and the protein expression pattern was analyzed using two-dimensional electrophoresis. Potential proteins involved in the response to water deficiency were identified by comparing the protein expression patterns under water deficit stress with the expression pattern in control conditions. The results revealed significant differences in root weight and root length at a 5% probability level, indicating the detrimental effects of water stress on plant roots. The proteomic analysis identified 98 reproducible protein spots, of which 10 exhibited statistically significant changes, with eight spots showing increased expression and one showing decreased expression. These protein spots were identified based on their molecular weight (MW) and isoelectric point (pI) through database searches. The identified proteins were classified into various functional categories related to stress response, including protein synthesis and accumulation, oxidative stress, response and defense against stress and metabolic pathways.

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References

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