Germination of Iranian and European barley cultivars under salinity stress and ‎its relationship with seed storage proteins

Maleki, Ali; Zarei, Leila; Bahraminejad, Sohbat; Cheghamirza, Kianoosh; Akbari, Leila; Fatahi, Fardin

doi:10.30473/cb.2023.69761.1932

Germination of Iranian and European barley cultivars under salinity stress and ‎its relationship with seed storage proteins

Document Type : Research Paper

Authors

Department of Plant Production and Genetics, Razi University, Kermanshah, Iran.

10.30473/cb.2023.69761.1932

Abstract

Germination is one of the most important stages of crop plant growth and proper germination in a wide range of environmental conditions is necessary for plant establishment. In this research, 20 varieties of Iranian barley and 24 varieties of European barley were used in three salinity levels (100, 200 and 300 mM sodium chloride) and a control level (distilled water) in order to investigate the tolerance of salinity stress in the germination stage, as a factorial test based on a completely randomized design with three replications in the mushroom cultivation chamber with controllable temperature and light and sterile conditions in the Nosoud agricultural service center by a protein marker in the biotechnology laboratory of the agriculture and natural resources of Razi university. The results of analysis of variance of traits related to germination showed that the effect of salinity, cultivar and salinity × cultivar were significant for all traits. By increasing the salinity level from 0 to 300 mM, all traits and indices decreased except the average germination time. The germination percentage had a positive and significant correlation with the total seedling length, shoot length, seed germination index, average germination rate and germination rate index. Stepwise regression analysis of different traits with protein markers showed that there were 13 bands associated with different traits. The most significant correlation with traits was related to the 60 kDa marker which was related four indices of seed germ, average germination speed, shoot length and cleoptile length. After the validation studies and confirmation of the results, the identified markers can be used in marker-assisted selection for related traits.

Keywords

Main Subjects

Biotic and Abiotic stress

References

Ahakpaz, F., Abdi, H., Neyestani, E., Hesami, Ali., Mohammadi, Behrouz., Nader Mahmoudi, K., Abedi-Asl, Gh., Jazayeri Noshabadi, M, Reza., Ahakpaz, F., & Alipour, H. (2021). Genotype-by-environment interaction analysis for grain yield of barley genotypes under dryland conditions and the role of monthly rainfall. Agricultural Water Management, 245, 106665. Al-Huqail, A. A. & Abdelhaliem, E. (2015). Evaluation of genetic variations in maize seedlings exposed to electric field based on protein and DNA markers. Biomedical Research International, 2015, 1-15. Alipour, S., Soltani, E., Alahdadi, I., Ghorbani Javid, M., & Akbari, Gh. (2022). Effect of planting date and salinity on biological stability of Salicornia iranica against fine dust stress. Journal of Crop Production, 15(1), 187-204 .(in Persian). Ashraf, M. (2010). Inducing drought tolerance in plants: recent advances. Biotechnology advances, 28(1), 169-183. Ashraf, Muhammad & Rauf, Humera. (2001). Inducing salt tolerance in maize (Zea mays L.) through seed priming with chloride salts: Growth and ion transport at early growth stages. Acta Physiologiae Plantarum, 23, 407-414. Bagheri, A, R., & Heydari Sharif Abad, H. (2016). Investigating the effect of drought stress on yield and yield components and ion content in bare barley (Hordeum sativum L.). Ecology of agricultural plants, 3(7-2), 1-15. Bajji, M., Kinet, JM.., & Lutts, S. (2002). Osmotic and ionic effects of NaCl on germination, early seedling growth, and ion content of Atriplex halimus (Chenopodiaceae). Canadian Journal of Botany, 80(3), 297-304. Bakhshayeshi Qashlaq, M., Kazemi Arbat, H., Sadeghzadeh Ahri, D., & Bakhshayeshi Qashlaq, H. (2013). Physiological effects of salt stress (sodium chloride) on germination and seedling growth of bread wheat genotypes. Agricultural applied research (research and development), 27(105), 119-126. Bouslama, M., & Schapaugh, W. T. (1984). Stress tolerance in soybean. I: Evaluation of three screening techniques for heat and drought tolerance. Crop Science, 24, 933- 937. Brar, G. S., Gomez, J. F., McMichael, B. L., Matches, A. G. & Taylor, H. M. (1991). Germination of twenty forage legumes as influenced by temperature. Agronomy journal, 83(1), 173-175. Chekani, U, al-Binin, Ajam Nowrozi, H., & Faghani, E. (2012). Evaluation of salinity tolerance of different genotypes of barley at the germination stage. Seed Research (Seed Science and Technology), 3(3), 69-75. Cherki, Gh., Foursy, Ahmed., & Fares, Kh. (2002). Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environmental and Experimental Botany, 47(1), 39-50. Dvoracek, V., Curn, V. & Moudry, J. (2003). Suitability of oat-seed storage-protein markers for identification of cultivars in grain and flour samples. Plant, Soil and Environment, 49(11), 486-491. Ellis, R, A., & Roberts, E. H. (1981). The Quantification of Ageing and Survival in Orthodox Seeds. Seed Science and Technology, 9, 373-409. Francia, E., Tacconi, G., & Crosatti, C. (2005). Marker assisted selection in crop plants. Plant Cell Tissue Organ Culture, 82, 317-342. Fufa, H., Baenziger, P. S., Beecher, B. S., Dweikat, I., Graybosch, R. A. & Eskridge, K. M. (2005). Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica, 145, 133-146. Ganj Ali, A., Parsa, H., & Hojjat, S. (2006). Genotypic diversity of root and aerial organ traits of chickpea seedlings (Cicer arietinum L.) in hydroponic and greenhouse environments. Iranian Agricultural Research Journal, 1, 143-155. Ghaffari Moghaddam, S., Sabouri, H., Abdollatif Gholizadeh, H., & Fallahi, A. (2019). Identification of QTLs associated with some (Hordeum vulgare L.) traits in a germination stage under salt stress conditions. Iranian Journal of Plant Biology, 11(3), 74 -94. (in Persian) Jahanbakhsh, S., Parmoon, Gh., and Azad, H., & Ghatei, A. (2019). Modeling hydrotime and threshold tolerance to salinity on germination different species Basil (Ocimum basilicum). Iranian Journal of Seed Science and Technology, 7(2), 119-142. Jalali, P., Navabpour, S., Yamchi, A., Soltanloo, H., & Bagherikia, S. (2020). Differential responses of antioxidant system and expression profile of some genes of two rice genotypes in response to salinity stress. Biologia, 75(5), 785-793. Jamil, M., Bae Lee, D., Yony Jun, K., Ashraf, M., & Chin, S. (2006). Effect of salt (NaCl) stress on germination and early seedling growth of four vegetable species. Journal Center Europ. Agriculture, 7, 273-282. Kafi, M., Barzoui, A., Salehi, M., Kamandi, A., Masoumi, A., & Nabati, J. (2017). Physiology of environmental stress in plants. Mashhad Academic Jihad Publications. 504 pp. Kakaei, M., & Kahrizi, D. (2011): Evaluation of seed storage protein patterns of ten wheat varieties using SDS-PAGE. Biharean Biologist 5, 116-118. Kakaei, M., Mazahery Laghab, H. & Kiani, S. (2014). Genetic variability of endosperm and embryo protein expression in some cultivated barley genotypes. Biharean Biologist, 8(1), 42-47. Khazaei, H. R., Nezami, A., Saadatian, B., Armand Pishe, O., & Pordel, R. (2016). Effect of seed priming on seedling growth of barely (Hordeumvulgare L.), under salinity stress in phytogel. Environmental Stresses in Crop Sciences, 9(1), 87-97. Langridge., P. (2018). Economic and academic importance of barley." In The barley genome, pp. 1-10. Leidi, E. O., Silberbush, M., & Lips, S. H. (1991). Wheat growth as affected by nitrogen type, pH and salinity. I. Biomass production and mineral composition. Journal of Plant Nutrition, 14(3), 235-246. Mascher, M., Gundlach, Heidrun, Himmelbach, A., Beier, Se., Twardziok, SO., Wicker, T., & Radchuk, V. (2017). A chromosome conformation capture ordered sequence of the barley genome. Nature, 544(7651), 427-433. Mehrabi Oladi, A, A., Omidi, M., & Fazli Nasab, B. (2018). Investigating the effects of salinity stress on seed germination, seedling growth and callus cultivation of rapeseed genotypes. Journal of Agricultural Plant Sciences of Iran, 42(1),81-90. Mostafavi, Kh., Golbashi, M., Izadi Darbandi, A., & Zarrabi, M. (2012). The effect of salinity stress and estimation of genetic parameters of some cultivars and lines of barley (Hordeum vulgare L.) in the stage of seed germination and early seedling growth. Iran seed science and technology, 1(2), 117-128. Munns, R. (2002). Comparative physiology of salt and water stress. Plant, Cell & Environment, 25, 239-250. Munns, R., Richard A. James, & Läuchli, A. (2006). Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany, 57(5), 1025-1043. Mwando, E., Angessa, T, Tolera., Han, Y., Zhou, G., & Chengdao Li. 2021. Quantitative trait loci mapping for vigour and survival traits of barley seedlings after germinating under salinity stress." Agronomy, 11(1), 103. Nawaz, M., Hassan, M. U., Chattha, M. U., Mahmood, A., Shah, A. N., Hashem, M., Alamri, S., Batool, M., Rasheed, A., Thabit, M. A., Alhaithloul, H. A. S., & Qari, S. H. (2022). Trehalose: a promising osmo-protectant against salinity stress-physiological and molecular mechanisms and future prospective. Molecular Biology Reports, 49(12), 11255-11271. Noroozi, M., Chavoshie, E., & Ghajar Sepanlou, M. (2022). Effect of Irrigation Water Salinity on Relative Yield and Some Morphological and Physiological Characteristics of Sorghum, Journal of Water Research in Agriculture (Soil and Water Sci.), 36(1), 55-74. (in Persian) Parvez., Shumaila, Abbas, Ghulam., Shahid, M., Amjad, M., Hussain, M., Ahmad Asad, S., Imran, M., & Asif Naeem, M. (2020). Effect of salinity on physiological, biochemical and photostabilizing attributes of two genotypes of quinoa (Chenopodium quinoa Willd.) exposed to arsenic stress. Ecotoxicology and Environmental Safety, 187, 109814. Ruan, CJ., Li, H., & Mopper, S. (2009). Characterization and identification of ISSR markers associated with resistance to dried-shrink disease in sea buckthorn. Molecular Breeding, 24, 255. Reynolds, M.P., Rebetzke. G., Pellegrineschi. A., & Trethowan, R. (2006). Drought adaptation in wheat. In: Ribaut, J.M. (ed.), Drought Adaptation in Cereals. New York, NY, USA: Food Products Press, pp. 401-446. Rui, M., Almeida, M., & Serralheiro, P., R. (2017). Soil salinity: effect on vegetable crop growth. Management practices to prevent and mitigate soil salinization. Horticulture, 3(2), 30. Saiz-Rubio, V., & Rovira-Más, F. (2020). From Smart Farming towards Agriculture 5.0: A Review on Crop Data Management. Agronomy, 10, 207. Soltani, A., Gholipoor, M., & Zeinali, E. (2005). Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 55(1-2), 195-200. Tricase, C., Lamonaca, E., Ingrao, C., Bacenetti, J., & Lo Giudice, A. (2018). A comparative Life Cycle Assessment between organic and conventional barley cultivation for sustainable agriculture pathways. Journal of Cleaner Production, 172, 3747-3759. Willenborg, Ch, J., C. Wildeman, J., K. Miller, A., G. Rossnagel, B., & J. Shirtliffe, S. (2005). Oat germination characteristics differ among genotypes, seed sizes, and osmotic potentials, Crop Science, 45(2), 2023-2029.