The Effect of Thermopriming on Transcriptional Response of Heat Stress Memory in Rapeseed

Taherifar, Navid; Taheri, Hengameh

doi:10.30473/cb.2024.70139.1946

The Effect of Thermopriming on Transcriptional Response of Heat Stress Memory in Rapeseed

Document Type : Research Paper

Authors

Navid Taherifar ¹
Hengameh Taheri ²

¹ 1. M.Sc. Student, Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

² Assistant Professor, Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

10.30473/cb.2024.70139.1946

Abstract

Heat stress has detrimental effects on the growth and performance of plants through biochemical, physiological, morphological, and molecular changes. Plants have developed complex mechanisms to balance growth and tolerance to stress, allowing them to effectively defend against more severe stresses by remembering mild stress and forming heat stress memory, known as thermopriming. To investigate the role of thermopriming in inducing the transcription response of HSFA2, HSFA1b and MIPS2 genes, the changes in the transcriptional level of the genes were studied at different times after priming and return stress in canola seedlings using the qRT-PCR technique. The results showed that the expression of these genes was not stable during the recovery period after the initial mild stress (memory phase), while their transcription level immediately after facing the second severe stress was induced at a much higher level in primed plants (P+T treatment) compared to non-primed plants (T treatment) which continued until 48 hours after return stress. Also, morphological analysis of seedlings at 7 and 14 days after release from the second stress showed that thermopriming increase the growth indices and heat tolerance in these plants through strengthening the expression of stress memory genes. Since the HSFA1b, HSFA2 and MIPS2 genes have maintained their expression level until days after the return stress, these genes can be the key components of the transcriptional memory of heat stress and be used in breeding programs and the development of heat tolerant varieties.

Keywords

Main Subjects

Biotic and Abiotic stress

References

AlKian Abadi, A., Taheri, H., & Sadr, A. S. (2023). Identification of key genes involved in the establishment and maintenance of heat stress memory in Arabidopsis seedlings using microarray data. Crop Biotechnology, 12(40), 63-81. Anckar, J., & Sistonen, L. (2011). Regulation of HSF1 function in the heat stress response, implications in aging and disease. Annual review of biochemistry, 80, 1089-1115. Balazadeh, S. (2022). A ‘hot’cocktail, The multiple layers of thermomemory in plants. Current Opinion in Plant Biology, 65, 102147. Bita, C. E., & Gerats, T. (2013). Plant tolerance to high temperature in a changing environment, scientific fundamentals and production of heat stress-tolerant crops. Frontiers in plant science, 4, 273. Brzezinka, K., Altmann, S., Czesnick, H., Nicolas, P., Gorka, M., Benke, E., ..., & Bäurle, I. (2016). Arabidopsis FORGETTER1 mediates stress-induced chromatin memory through nucleosome remodeling. elife, 5, e17061. Charng, Y. Y., Liu, H. C., Liu, N. Y., Chi, W. T., Wang, C. N., Chang, S. H., & Wang, T. T. (2007). A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant physiology, 143(1), 251-262. Crisp, P. A., Ganguly, D. R., Smith, A. B., Murray, K. D., Estavillo, G. M., Searle, I., ..., & Pogson, B. J. (2017). Rapid recovery gene downregulation during excess-light stress and recovery in Arabidopsis. The Plant Cell, 29(8), 1836-1863. Crisp, P. A., Ganguly, D., Eichten, S. R., Borevitz, J. O., & Pogson, B. J. (2016). Reconsidering plant memory, Intersections between stress recovery, RNA turnover, and epigenetics. Science advances, 2(2), e1501340. Fragkostefanakis, S., Mesihovic, A., Simm, S., Paupière, M. J., Hu, Y., Paul, P., ..., & Scharf, K. D. (2016). HsfA2 controls the activity of developmentally and stress-regulated heat stress protection mechanisms in tomato male reproductive tissues. Plant physiology, 170(4), 2461-2477. Friedrich, T., Oberkofler, V., Trindade, I., Altmann, S., Brzezinka, K., Lämke, J., ..., & Bäurle, I. (2021). Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis. Nature Communications, 12(1), 3426. Hasanuzzaman, M., Nahar, K., Alam, M. M., Roychowdhury, R., & Fujita, M. (2013). Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. International journal of molecular sciences, 14(5), 9643-9684. Hemme, D., Veyel, D., Mühlhaus, T., Sommer, F., Jüppner, J., Unger, A. K., … and Schroda, M. (2014). Systems-wide analysis of acclimation responses to long-term heat stress and recovery in the photosynthetic model organism Chlamydomonas reinhardtii. The Plant Cell, 26(11), 4270-4297. Hilker, M., Schwachtje, J., Baier, M., Balazadeh, S., Bäurle, I., Geiselhardt, S., ..., & Kopka, J. (2016). Priming and memory of stress responses in organisms lacking a nervous system. Biological Reviews, 91(4), 1118-1133. Hu, S., Ding, Y., & Zhu, C. (2020). Sensitivity and responses of chloroplasts to heat stress in plants. Frontiers in Plant Science, 11, 375. Jaskiewicz, M., Conrath, U., & Peterhänsel, C. (2011). Chromatin modification acts as a memory for systemic acquired resistance in the plant stress response. EMBO reports, 12(1), 50-55. Kappel, C., Friedrich, T., Oberkofler, V., Jiang, L., Crawford, T., Lenhard, M., & Bäurle, I. (2023). Genomic and epigenomic determinants of heat stress-induced transcriptional memory in Arabidopsis. Genome Biology, 24(1), 1-23. Lämke, J., Brzezinka, K., Altmann, S., & Bäurle, I. (2016). A hit‐and‐run heat shock factor governs sustained histone methylation and transcriptional stress memory. The EMBO journal,35(2),162-175. Ling, Y., Serrano, N., Gao, G., Atia, M., Mokhtar, M., Woo, Y.H., Bazin, J., Veluchamy, A., Benhamed, M., & Crespi, M. (2018). Thermopriming triggers splicing memory in Arabidopsis. Journal of Experimental Botany, 69(10), 2659-2675. Liu, H.C., Lämke, J., Lin, S.Y., Hung, M.J., Liu, K.M., Charng, Y.Y., & Bäurle, I. (2018). Distinct heat shock factors and chromatin modifications mediate the organ‐autonomous transcriptional memory of heat stress. Plant Journal, 95(3), 401-413. Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25(4), 402-408. Masson-Delmotte, V., Zhai, P., Pörtner, H. O., Roberts, D., Skea, J., & Shukla, P. R. (2022). Global Warming of 1.5 C, IPCC special report on impacts of global warming of 1.5 C above pre-industrial levels in context of strengthening response to climate change, sustainable development, and efforts to eradicate poverty. Cambridge University Press. Moore, C. E., Meacham-Hensold, K., Lemonnier, P., Slattery, R. A., Benjamin, C., Bernacchi, C. J., ..., & Cavanagh, A. P. (2021). The effect of increasing temperature on crop photosynthesis, from enzymes to ecosystems. Journal of Experimental Botany, 72(8), 2822-2844. Nover, L., Bharti, K., Döring, P., Mishra, S. K., Ganguli, A., & Scharf, K. D. (2001). Arabidopsis and the heat stress transcription factor world, how many heat stress transcription factors do we need?. Cell stress & chaperones, 6(3), 177. Ohama, N., Sato, H., Shinozaki, K., & Yamaguchi-Shinozaki, K. (2017). Transcriptional regulatory network of plant heat stress response. Trends in plant science, 22(1), 53-65. Oyoshi, K., Katano, K., Yunose, M., & Suzuki, N. (2020). Memory of 5-min heat stress in Arabidopsis thaliana. Plant Signaling & Behavior, 15(8), 1778919. Pfaffl, M. W., Horgan, G. W., & Dempfle, L. (2002). Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic acids research, 30(9), e36-e36. Yeh, C. H., Kaplinsky, N. J., Hu, C., & Charng, Y. Y. (2012). Some like it hot, some like it warm, phenotyping to explore thermotolerance diversity. Plant Science, 195, 10-23. Yoshida, T., Ohama, N., Nakajima, J., Kidokoro, S., Mizoi, J., Nakashima, K., & Yamaguchi-Shinozaki, K. (2011). Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression. Molecular Genetics and Genomics, 286, 321-332.