Estimating breeding value of the morphological traits in oilseed sunflower genotypes under normal and drought stress conditions with microsatellite and retrotransposon based markers

Document Type : Research Paper

Authors

1 Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran.

2 Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract

Knowledge on genes effect and action (additive/dominance) is one of the necessities to achieve cultivars with high performance and quality. Estimating the breeding value (additive effect) can be done thanks to molecular markers through best linear unbiased prediction (BLUP). In the present study, 100 oilseed sunflower genotypes were evaluated based on the 10×10 lattice design during two crop years of 1392-1393 under normal and drought stress (irrigation limitation) conditions. The breeding value of 13 traits in 78 genotypes out of 100 was estimated due to having genotyping data with SSR and Retrotransposon based markers in each one of normal and drought stress (irrigation limitation) conditions through BLUP. For this purpose, the kinship matrix was calculated by SSR and Retrotransposon based markers data. According to total ranks of breeding values of all studied traits estimated by molecular data of both markers, in normal conditions, genotypes 47, 11, 8 and 35 and under drought stress (irrigation limitation) conditions, genotypes 8, 11 and 35 showed the highest breeding value. Based on SSR markers data in normal conditions; genotypes 76, 36, 34 and 41 and based on Retrotransposon based markers data; genotypes 61, 78, 72 and 52, and in drought stress (irrigation limitation) conditions based on SSR markers data; genotypes 76, 38, 34, 29 and 70 and based on Retrotransposon based markers data; genotypes 16, 71, 78 and 61 showed the lowest breeding value. Considering both studied conditions and all studied traits and both molecular markers information, genotypes 8, 11 and 35 with high breeding value are introduced as desirable parents for breeding programs.

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