Identification and Isolation of Transcripts in Response to KCl from Aeluropus littoralis

Fatemi Ardestani, Seyyedeh Farzaneh; Nematzadeh, Ghorbanali; Askari, Hossein; Hashemi, Hamidreza

Identification and Isolation of Transcripts in Response to KCl from Aeluropus littoralis

Document Type : Research Paper

Authors

¹ M.Sc. of Plant Breeding, Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari, Sari, Iran,

² Professor of Biotechnology, Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agriculture and Natural Resources of Sari, Sari, Iran,

³ Assistant Professor, Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, Tehran, Iran

⁴ M.Sc. of Plant Breeding, Genetic and Agricultural Biotechnology Institute of Tabarestan, University of Agricultural Sciences and Natural Resources of Sari, Sari, Irann

Abstract

Soil salinity limits crop production by creating osmotic stress and disruption of ion homeostasis, leads to damage at the molecular level and finally cell death. In this study, gene expression analysis based on cDNA-AFLP technique was used to compare the expression profiles of KCl stress at three levels: 0 (control), 200mM and 400mM, in Aeluropus littoralis which is the closest family to cereal. Among 34 isolated ESTs, 27 ESTs were obtained with the average length of 280 bp. The nucleotide sequences were compared with those in the GenBank database. Approximately 80% of the ESTs show homology to nucleotide or amino acid sequences in the GenBank database and 6 ESTs show no significant similarity in the GenBank database which considered as novel genes. Finally, 27 ESTs were recorded in NCBI database which are included potassium transporter, ribosomal protein, NADH dehydrogenase and golgin. The result of this research is very important to understand molecular basis and resistance mechanisms of drought stress for breeding and genetic engineering to improve crop resistance against stress and the production of resistant plants. EST classification based on responses to stress, will facilitate performance analysis, characterization of responsive genes in plant roots of Aeluropus littoralis to stress in future studies on this herb that is a member of the Poaceae family.

Keywords

20.1001.1.22520783.1393.4.6.7.1

Main Subjects

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