Characterization of new male sterile rice lines and validation of linked molecular markers for identification of their genetic seed purity

Tarang, Alireza; Sattari, Majid

doi:10.30473/cb.2024.70720.1959

Characterization of new male sterile rice lines and validation of linked molecular markers for identification of their genetic seed purity

Document Type : Research Paper

Authors

Alireza Tarang ¹
Majid Sattari ²

¹ Rice Research Institute of Iran (RRII), Agricultural Research,Education and Extension Organization (AREEO), Rasht, Iran

² Rice Research Institute of Iran (RRII), Agricultural Research,Education and Extension Organization (AREEO), Amol, Iran

10.30473/cb.2024.70720.1959

Abstract

The use of heterosis in hybrid rice breeding aims to increase productivity, with hybrid varieties yielding 20 to 30 percent more than conventional varieties. Maintaining genetic purity in hybrid seed production is critical to prevent contamination and selfing, especially in cytoplasmic male sterile lines. This study aimed to identify microsatellite markers to distinguish hybrid rice parental lines and to assess seed purity and characterize the morphology of F1 hybrid rice. Initial trait assessments, including outcrossing levels and sterility stability, are critical for selecting preferred male sterile lines. Eighteen Iranian and exotic cytoplasmic male sterile lines and one thermosensitive genetic male sterile line, were evaluated for various traits in a randomized complete block design. PCR-based molecular markers were used to assess purity, revealing significant trait differences among lines. Selection and prioritization of superior lines such as Fajr A, Neda A and IR58025 A was based on fertility percentage, panicle yield and other traits. The Dasht B line with favorable traits such as proper height and panicle length proved to be a promising candidate for seed production. Molecular tests with PCR-based markers effectively discriminated between male sterile and conservation lines. The drrcms marker showed promise in distinguishing cytoplasmic male sterility lines from different sources, offering potential for genetic purity evaluation and infection testing of seed parents. These results point to valuable alternatives for maintaining genetic integrity in hybrid rice production.

Keywords

Main Subjects

Molecular Plant Breeding

References

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