Structure and similarity of dehydrin proteins using bioinformatics tools in monocotyledons ‎and dicotyledons plants

Saed-Moucheshi, Armin; Shirkhani, Ali

doi:10.30473/cb.2024.70852.1962

Structure and similarity of dehydrin proteins using bioinformatics tools in monocotyledons ‎and dicotyledons plants

Document Type : Research Paper

Authors

Crop and Horticulture Reseach Department, Kermanshah Agricultural and Natural Resources Research and Education Center (AREEO), Kermanshah, Iran.

10.30473/cb.2024.70852.1962

Abstract

Dehydrin (DHN) proteins are a group of proteins that effectively respond to abiotic stresses such as cold and drought stress in plants. These proteins are a subset of protective greater group of proteins called type II Late embryogenesis abundant which are protecting other proteins from stresses shock. Due to the significant effect of dehydrin proteins in plants, specialy under abiotic stresses, the aims of this study were to survey the linear structure along with phylogeny relationship of this proteins’ group in different plants species. The protein linear sequences of different plant species were downloaded from NCBI site and then were aligned using MegaX software. The results of aligning showed highly conserved segments within the considered sequences such as K- and S-segments that are respectively responsible for covering other proteins and protecting them from damaging effects of stresses and transporting dehydrin proteins from cytoplasm to nucleus. Using the sequences’ alignment, phylogenic tree was extracted using Neighbor joining method. Furthermore, linear sequence order of amino acids and their ratio in the structure of these protein were evaluated. Folowing that, the composition of these proteins genomic sequences were considered to compare with the results of amino acids evaluation. The results indicated that dicotyledon and monocotyledon plants can be clearly separated into two distinguished classes based on the amino acid structure of DHN proteins. Similarly, The ratio and order of DNHs linear sequences were distinctly altered between mono- and di-cotyledon plants. Evaluation of genomic base pairs of these proteins showed that there are numerous unchanged motifs with eighter no or a little difference shared among the genomic sequences of DHN proteins.

Keywords

Main Subjects

Bioinformatics

References

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