genetic diversity of the green oak leaf roller moth, Tortrix viridana (Lep., Tortricidae) in Zagros oak forests using s rDNA28

Mousivand, Rezvan; Majdi, Mohammad; Fatehi, ّFoad; Ghobari, Hamed

doi:10.30473/cb.2023.69145.1926

genetic diversity of the green oak leaf roller moth, Tortrix viridana (Lep., Tortricidae) in Zagros oak forests using s rDNA28

Document Type : Research Paper

Authors

¹ University of KurDepartment of plant production and Genetics, University of Kurdistan, Sanandaj Irandistan

² Department of Agriculture, Payame Noor University, Tehran, Iran

³ Department of plant Protection, University of Kurdistan, Sanandaj Iran

10.30473/cb.2023.69145.1926

Abstract

the genetic diversity of the T. viridana on the host oak species Quercus branti was investigated using the 28s gene sequence in some Zagros forests. The samples were collected from the forest area of West Azarbaijan, Lorestan, Kurdistan and Kermanshah provinces. The Collected samples, which were in the larval stage, were grown in laboratory conditions until they turned into paup and then into complete insects, for DNA extraction. DNA extraction was done by CTAB method. 21 samples were selected to investigate genetic diversity using the 28s gene, and 18 sequences DNAs were of suitable quality for further investigations. In order to amplify 28s region, based on the sequence obtained from the NCBI site.The desired region in the collected samples was amplified using the PCR method and the resulting product was sequenced. The sequences were edited using Bioedit software and aligned with the help of MegaX software, and the phylogenetic tree was drawn by UPGMA method with 1000 sampling repetitions. The results of this study, based on the phylogenetic tree, showed that the different samples of the oak saplings have genetic diversity based on the geographical distance. Assessment of genetic structure of the populations showed that higher diversity was demonstrated in intrapopulations than interpopulations geographically. 28s gene showed the presence of 5 haplotypes in these populations. The presence of low diversity between populations indicated the existence of gene flow between populations as well as genetic compatibility in the studied populations.

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