Study of changes in the phenylalanine ammonia-lyase 2 (PAL2) and thaumatin-like protein (TLP) genes transcripts in sunflower genotypes in response to fungi isolates of Black stem disease

Mohamadian Farsani, Arezo; Hatami Maleki, Hamid; Darvishzadeh, Reza; Hoshyardel, Faramarz

Study of changes in the phenylalanine ammonia-lyase 2 (PAL2) and thaumatin-like protein (TLP) genes transcripts in sunflower genotypes in response to fungi isolates of Black stem disease

Document Type : Research Paper

Authors

¹ Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.

² Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

Abstract

Black stem disease is one of the most important fungi diseases of sunflower. The use of resistant genotypes is potentially one of the economical ways for its control. In this study, expression level of genes including phenylalanine ammonia-lyase 2 (PAL2) and thaumatin-like protein (TLP) were measured in sunflower genotypes including ENSAT-B5, AS613 and mutant genotype M5-54-1 infected with MA6, MP8 and MP10 isolates of Phoma macdonaldii via quantitative RT-PCR technique. Results revealed that transcript levels of the both PAL2 and TLP genes were significantly affected by isolate and genotype and genotype-isolate interactions. In this study, the expression levels of genes PAL2 and TLP showed higher increase against the MP8 isolate. Among studied genotypes, genotype ENSAT-B5 possessed highest increasing in expression levels of genes PAL2 and TLP. The resistant and susceptible genotypes had possessed high and low transcript levels of PAL2 and TLP and disease symptoms were seen in susceptible genotypes.

Keywords

20.1001.1.22520783.1393.4.7.2.8

Main Subjects

References

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