Design, fabrication and the use of temporary immersion system for micropropagation in Damask rose (Rosa damascena Mill.)

Document Type : Research Paper

Authors

1 M.Sc., Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran,

3 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

4 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Malayer University, Malayer, Iran

Abstract

The plant bioreactors can be used for in vitro plant propagation and leading to a decrease in the costs of micropropagation. In this research, a simple bioreactor including a temporary immersion system was designed and made for Damask rose micropropagation. To optimize this system, the effects of the tube type, container size and different substrates were investigated in the independent experiments. Also, the temporary immersion system was compared with the solid and liquid media. Results showed that the highest number of the shoots in the temporary immersion system were obtained by the use of the white tube (11 shoots per explant), 600 ml glass container (10.36 shoots per explant) and without any substrate (10.76 shoots per explant). Also, temporary immersion system was significantly better than the solid and liquid media for shoot production (10.66, 2.56 and 5.66, respectively).

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References

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