Application of microsatellite markers and some morphological traits in core collection identification of Prunus dulcis Mill.

Document Type : Research Paper

Authors

1 Associate Professor, Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran

2 M.Sc. Agricultural Biotechnology, Payame Noor University, Tehran, Iran

3 Assistant Professor, Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran

4 Associate Professor, Department of Horticulture,Seed and Plant Improvement Institute, Karaj, Iran

Abstract

A core collection consists of a set of accessions selected to represent the genetic diversity of a base collection. This strategy was introduced with the intention of minimizing the cost of genetic resource conservation, while ensuring maximum genetic variation. It also allows a rapid evaluation of germplasm, and a better access to the base collection.In this study, One hundred and thirty-nine accessions of almond with diverse origins were used for evaluation of genetic diversity and core collection establishment using 32 simple sequence repeat loci and some quantitative and qualitative traits. A total of 252 alleles were detected, which average allelic richness was 7.75 alleles per locus. The model-based structure analysis here revealed the presence of five subpopulations in the base set, which was approximatelyconsistent with clustering based on the genetic distance. Using a heuristic approach, a core set of 112 and 20 accessions was successfully developed using molecular and morphological traits respectively which showed 100% coverage of alleles with minimum redundancy. The results from molecular analyses did not correspond to morphological traits, hence to establish of core collection we have to study both morphological and molecular markers, simoltaneously. These results will provide an effective aid for future allele mining, association genetics, mapping and cloning gene(s), germplasm conservation, and promotion in breeding programs at future.

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References

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