Mapping QTLs associated with wheat seed germination under normal and drought stress conditions

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Agriculture, Payame Noor University, Tehran, Iran.

2 Professor, Department of Plant Breeding, University of Tabriz, Tabriz, Iran

Abstract

Seed germination (capacity, time, and synchronization) is a manifest characteristic of the standard germination test of a cultivated variety. In order to identify genomic regions associated with seed germination, 149 bread wheat recombinant inbred lines and their parents RojoYecora and No. 49 were conducted  in an alpha lattice design with two replications at the University of Mahabad during 2013-14, under normal and moisture stress conditions. Different traits including index of germination speed, final germination percentage, germination speed, germination rate, and average daily germination were measured. In this study, 340 pairs of microsatellite primers were used to determine the parental polymorphism. Furthermore, 7 retrotransposon-based markers on LTR regions of barely retrotransposons, and their twenty-eight combinations at IRAP technique, as well as, 63 combination of this primer with anchored ISSR primers at REMAP technique were used for population screening. QTL analysis using composite interval mapping (CIM) for each trait in each environment and mean of two environments were accomplished. For all studied traits, transgressive segregation was observed. In general, eleven QTLs were detected for all traits. Total phenotypic variance explained by these QTLs varied from 11.82 to 21.42 percent. The highest LOD value for QTL controlling germination speed (LOD =6.65)  was obtained on  chromosome 4BQSg-Normal. The results revealed that QTLs of index of germination rate and germination speed related to the quantity and quality of seed germination had desirable performance in all three environments. However, detected stable and cluster QTLs may be used in marker assisted selection (MAS) programs.

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References

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