Optimization of callus induction and regeneration in two Indica rice (Oryza sativa L.) varieties under absence of proline to decline its undesirable effects on 2-acetyl-1-pyrroline and other aromatic compounds

Document Type : Research Paper

Authors

1 Ph.D, Cytogenetic Lab, Department of Botany, University of Pune, Pune, India

2 Associate Professor, Department of Botany, University of Pune, Pune, India.

3 Professor, Department of Agriculture, Payeme Noor University, Tehran, Iran.

Abstract

In most transformation studies it has been indicated that adding proline to the tissue culture medium can increase the callus induction frequency and reduce induction time. Adding proline to callus induction medium in this phase can affect the production of aromatic compounds in rice and if the goal of exogenous gene transformation is increasing the rice aromatic associated secondary metabolites such as 2-acetyl-1-pyrroline (2AP), this amino acid can slipup the final results. Although, absence of amino acids such as proline can reduce callus induction percentage. In this research, callus induction from two indica rice varieties; Ambemohar 157 and Indrayani were optimized, using MS medium having various concentrations of 2,4-D, without using proline. The results revealed that 2.5 mgl-1 of 2,4-D for Ambemohar 157 and 4 mgl-1 for Indrayani can lead to better callus induction. These results indicated that absence of proline can be disregard by increasing of 2,4-D concentrations. Calluses obtained from the best hormone treatment were cultured on MS fortified with 0.01 mgl-1 NAA + (1, 2, 3, 4, 5) mgl-1 BAP for shoot regeneration. The highest percentage of regeneration was achieved on MS supplemented with 2 mgl-1and 3 mgl-1 for Ambemohar157 and Indrayani cultivar respectively. Proline contents in calli which were growth in MS medium supplemented with 500 mg/L of proline showed approximately 12 to 14 fold increase over the calli growth in non-proline added medium. 

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References

AbeT, Futsuhara YG (1986) Genotypic variability for callus formation and plant regeneration in rice. Theoretical and Applied Genetics 72: 3-10.
Ali SS,Jafri SJ, Khan MJ,  ButtMA (1993) Inheritance studies on aroma in two aromatic varieties of Pakistan. International Rice Research Newsletter 18(2): 6-7.
Ayers NM, Park WD (1994) Genetic transformation of rice. Critical Rev. Plant Science 13: 219-239.   
Bates  L (1973) Rapid determination of free proline for water stress studies. Plant Soil 39: 205- 207.
Buttery RG, Ling L., Juliano B O (1982) 2-acetyl-1-pyrolline; an important aroma component of cooked rice. Chem. Ind. (London) 23: 958-959.       
ChandS ,Sahrawat  A  K (2001) Stimulatory effect of partial desiccation on plant regeneration in indica rice (Oryza sativa L.). Journal of Plant Biochemistry and Biotechnology 10: 43-47.
Ge X, Chu Z, Lin Y, Wang S (2006) A tissue culture system for different germplasms of indicarice. Plant Cell Report 25: 392-402.
Huang CT, Teng SC, Chang LJ, Chuang SH, Ho T C, Wu LM (2008) Biosynthetic mechanism of 2-Acetyl-1-pyrroline and Its relationship with Δ1-pyrroline-5-carboxylic acid and methylglyoxal in aromatic rice (Oryza sativa L.) Callus. Journal of Agriculture Food Chemistry. 56: 7399-7404.
Huang CT, Huang YW, Hung HJ, Ho CT, Wu ML (2007) ∆¹-Pyrroline-5-carboxylic Acid Formed by Proline Dehydrogenase from the Bacillus subtilis ssp. natto Expressed in Escherichia coli as a Precursor for 2-Acetyl-1-pyrroline. Journal of Agriculture and Food Chemistry 55: 5097-5102.
Kaikavoosi K (2012) Overexpression of proline synthesizing gene for increasing aroma quality in non-basmati scented rice (Oryza sativa L.) through genetic transformation. Ph.D dissertation, Department of Botany, University of Pune, Pune, India.
Kaikavoosi K, Nadaf AB (2012) Aroma augmentation in indica rice varieties through genetic transformation and its analysis using headspace-solidphasemicroextraction (HS-SPME) coupled with GC-FID). In the International Conference on Business Opportunities in Life Sciences, Pune, India, 28-30 January, pp: 44-45.
Kathuria H, Giri J, Tyagi H, Tayagi AK (2007) Advances in transgenic rice biotechnology. Critical Reviews in Plant Sciences 26: 65-103.         
KaviKishor PB, Hong Z, Miao G, Chein H, Verma, D (1995) Overexpression of ∆¹-pyrroline-5-Carboxylate synthetase increases proline production and confers osmo tolerance in transgenic plants. Plant Physiology 108: 1387-1394.
Khanna HK, Raina SK (1998) Genotype and culture media interaction effects on regeneration response of three indica cultivars. Plant Cell, Tissue Organ Culture 52: 145-152.
Kumar KK, Muruthasalam S, Loganathan M, Sudhaka, Balasubramanian P (2005) An improved Agrobacterium-mediated transformation protocol for recalcitrant elite indica rice cultivars. Plant Molecular and  Biology  Report 23: 67-73.
Kumar V, Shriram V, KaviKishor PP, Jawali N, Shitole MG (2010) Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene. Plant Biotechnology Report. 4: 37-48.
Lin YZ, Zhang Q (2005) Optimizing the tissue culture conditions for high efficiency transformation of indica rice. Plant Cell Report 23: 540-547.     
Martinez TM, Cabrera PJL Herrera EL (2003) Improvement of Rice Transformation Using Bombardment of Scutellum-Derived Calli. Plant Molecular Biology Report. 21: 429-437.
Mathure S, Wakte KV, Jawali N, Nadaf AB (2011) Quantification of 2-Acetyl-1-pyrroline and Other Rice Aroma Volatiles Among Indian Scented Rice Cultivars by HS-SPME/GC-FID. Food Analytical Methods 2011; 4(3):326-333.
Mezl VA, Knox W (1976) Properties and analysis of a stable derivative of pyrroline-5-carboxylic acid for use in metabolic studies. Analytical Biochemistry. 74: 430-440.
Mohanty A, Sarma NP, TyagiAK (1999) Agrobacterium-mediated high frequency transformation of an elite indica rice variety Pusa Basmati 1 and transmission of the transgenes to R2 progeny. Plant Science. 147: 127-137.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Plant Physiology 15: 473- 497.  
Naik SD (2011) In vitro expression analysis of 2-acetyl-1-pyrrolin in scented rice (Oryza sativa L.). M. Phil dissertation, Department of Botany, University of Pune, Pune, India.
Nancy L, Yunzhu W, Jinshui Y, Koulin G, Shu H, Jiazhen T, Douglas T (1991) Efficient transformation and regeneration of rice small cell groups. Proc. Natural Academic Science Journal of USA 88: 6389-6393.
Peng J, Hodges TK (1989) Genetic analysis of plant regeneration in rice (Oryza sativa L.). In Vitro Cellular and Developmental Biology 25: 91-94.
Rachmawati D, Anzai H (2006) Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars. Plant Biotechnology. 23: 521-524.
Rashid H, Abbasi FM, Quraishi A (2003) Plant Regeneration from Seed Derived Callus of three varieties of Basmati Rice. Plant Tissue Culture Journal. 13(1): 75-79.
Romanczyk LJ Jr, McClelland LS, Aitken WM (1995) Formation of    2-Acetyl-I-pyrroline by Several Bacillus cereus Strains Isolated from Cocoa Fermentation Boxes. Journal of Agriculture and Food Chemistry. 43: 469-475.
Sadhukhan RN, Roy K, Chattopadhyay P (1997) Inheritance of aroma in two local aromatic rice cultivars. Environment and Ecology. 15(2): 315-317.
Sahi C, Sing A, Blumwald E, Grover A (2006) Beyond osmolytes and transporters: novel plant salt-stress tolerances-related genes from transcriptional profiling data. Plant Physiology. 127: 1-9.     
Sridevi G, Dhandapani M, Veluthambi K (2005) Agrobacterium-mediated transformation of White Ponni, a non-basmati variety of indicarice (Oryza sativa L.) Current Science. 88 (1): 128-132.           
Summart J, Panichajakul S, Prathepha P, Thanonkeo P (2008) Callus Induction and Influence of Culture Condition and Culture Medium on Growth of Thai Aromatic Rice, “KhaoDawk Mail 105”, Cell Culture. World Applied Sciences Journal. 5(2): 246-251.   
Suprasanna P, Ganapathi TR, Ramaswamy NK, Surendranathan  K, Rao PS (1998) Aroma synthesis in cell and callus cultures of rice. In Rice Genetics, Proceedings of the International Rice Genetics Symposium, International Rice Research Institute. 15: 123-125. 
Wakte VK, Patil GB, Nadaf AB (2005) Tissue culture technique as a tool in micropropagation, synthesis of aroma compounds and conservation of local scented non-basmati type rice varieties (Oryza sativa L.). In Karuppay SM and Gacche RN (eds). The National Conference on Bioactive Compounds: new frontiers and therapeutic usage, Nanded, India, 25-26 Feb., pp: 7-16.
Wakte VK, Kad T, Zanan RL, Nadaf AB (2011) Mechanism of 2-acetyl-1-pyrroline biosynthesis in BassialatifoliaRoxb. Flowers. PhysiolMolBiol Plants. (July–September 2011) 17(3): 231-237.
Wu  ML, Chou KL, Wu  CR, Chen JK, Huang  TC (2009) Characterization and the Possible Formation Mechanism of 2-Acetyl-1-Pyrroline in Aromatic Vegetable Soybean (Glycine max L.). Journal of Food Science. 74: 192-197.
Yamchi A, Jazii RF, Ghobadi C, Mousavi A, Karkhanehee AA (2005) Increasing of tolerance to osmotic stresses in tobacco Nicotianatabacumcv. Xanthi through Overexpression of P5CS Gene. Journal of Science and technology of Agriculture and Natural Resources. Isfahan University, Iran 8(4): 31-40.
Yoshihashi TN, Huong TT, Inatomi H (2002) Precursors of 2-acetyl-1-pyrroline, a potent flavor compound of an aromatic rice variety. Journal of Agricultural Food Chemistry. 50: 2001-2004.
Zhang L, Hattori K, Zhang L (1996) Genetic analysis of regeneration ability in rice seed callus. Genes and Genetics Systems. 71: 313-317.

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