Silicon role in molecular activity of barley antioxidant genes under drought stress

Heidari, Roya; Haddad, Raheem

Silicon role in molecular activity of barley antioxidant genes under drought stress

Document Type : Research Paper

Authors

Roya Heidari ¹
Raheem Haddad ²

¹ M.Sc. in Agricultural Biotechnology, Imam Khomeini International University. Qazvin, Iran

² Associated Professor, Agricultural Biotechnology Department, Imam Khomeini International University, Qazvin, Iran.

Abstract

Silicon is accounted as one of the elements that induces resistance to different kinds of stresses especially drought stress in plants. In this study, the effect of silicon was analyzed on the activity of antioxidant enzymes Catalase, Ascorbate peroxidase and Superoxide dismutase and also related genes expression pattern and H2O2 content on two varsities, NIMROOZ (drought resistant) and GORGAN (semi-resistant) under three treatments of drought, silicon-drought and control in a completely randomized design with three replications in four leaf developmental stage in a green house. Based on the results of factorial test, catalase activity was decreased in semi-sensitive varity and silicon treatment increased such activity in both varieties under drought stress. While the activity of two other enzymes (Ascorbat peroxidase and Superoxide dismutase) was increased under drought stress and silicon treatment intensified their activity in comparison with control. Their pattern of gene expression changed in a similar way of enzyme activity. It might be concluded that, the role of silicon in enhancing drought tolerance in barley plant is because of increasing activity of antioxidant enzymes and expression of stress responsible genes, and therefore, causes reduction in activity of reactive oxygen spices produced under drought stress.

Keywords

20.1001.1.22520783.1394.5.9.6.3

Main Subjects

Biotic and Abiotic stress

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