Evaluation of chitinase and β-1,3glucanase expression profile in Pseudomonas flourescens and salicylic acid treated wheat against Septoria tritici bloch (STB)

Document Type : Research Paper

Authors

1 Ph.D. student, Department of Plant Pathology, Tarbiat Modares University, Faculty of Agriculture, Tehran, Iran

2 Assistant Professor, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

3 Professor, Department of Plant Pathology, Tarbiat Modares University, Faculty of Agriculture, Tehran, Iran

4 Associate Professor, Department of Plant Pathology, Tarbiat Modares University, Faculty of Agriculture, Tehran, Iran

Abstract

Induced resistance is one the ways by which plants cope with the biotic stresses. Pathogenesis-related proteins (PRs), including chitinase and β-1,3-glucanase are synthesized by plant in response to pathogenic infection. In present work, we have carried out inoculation experiment in wheat to investigate the effect of salicylic acid (SA) and Pseudomonas flourescens on Septoria tritici Bloch (STB) symptoms. Subsequently, the expression profiles of chitinase and β-1,3-glucanase were analyzed with semi-quantitative RT-PCR at 0, 4, 14 and 24 days after inoculation in both treated and control plants. Results showed that the expression of both chitinase and β-1,3-glucanase genes increased in interaction with Z. tritici and the application of SA and P. flourescens. On the other hand, the application together had no significant effect on gene expression. Briefly, it can be concluded that increment of chitinase and β-1,3-glucanase expression level with the application of SA and P. flourescens would play role induction of plant defense against STB.

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References

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