Investigation of differential protein expression of protective proteins under salinity stress in Arabidopsis by iTRAQ-2DLC-MS/MS and analysis of gene expression and related gene network

Baghery, Mohammad Amin; Najafi Zarini, Hamid

Investigation of differential protein expression of protective proteins under salinity stress in Arabidopsis by iTRAQ-2DLC-MS/MS and analysis of gene expression and related gene network

Document Type : Research Paper

Authors

¹ Ph.D Student of Plant Breeding, Sari Agriculture Sciences and Natural Resources University (SANRU), Sari, Iran

² Assistant Professor of Plant Biotechnology, Sari Agriculture Sciences and Natural Resources University (SANRU), Sari, Iran.

Abstract

Identification and quantification of proteins and genes that expressed in the salinity stress conditions could lead to better understanding of the response mechanisms. So, in this study iTRAQ comparative proteomic method was used to investigate the protein changes and quantification of them under salinity stress. Then, more studies about the function of identified genes were done using gene expression and co-expression data obtained from databases and analysis of related sequences. According to the results of iTRAQ-2DLC-MS/MS a set of proteins including P5CS1, KIN1, KIN2, ERD10, ERD14 and COR47 were identified. Results showed that expressions of the related genes were not just restricted to salinity stress. They were also involved in other osmotic stresses. Gene network based on identified proteins was evaluated by String software. Based on the results, during the expression of these genes, protective compounds such as compatible solutes, dehydrin proteins and etc. were produced in the cells. Presence of the compounds led to induction of resistance and tolerance mechanisms in plants against osmotic stress such as salinity.

Keywords

20.1001.1.22520783.1394.5.11.4.5

Main Subjects

Bioinformatics

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