Studying genetic variation of sunflower mutant lines and identification of AFLP markers associated with seedling characteristics under salinity stress conditions

Hatami Maleki, Hamid; Jannatdoust, Marjan; Darvishzadeh, Reza

Studying genetic variation of sunflower mutant lines and identification of AFLP markers associated with seedling characteristics under salinity stress conditions

Document Type : Research Paper

Authors

¹ Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

² M.Sc. in Agricultural Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran

³ Professor, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran

Abstract

Salinity stress is one of the abiotic stresses which threaten plant species. Achieving any progress in breeding for tolerance to stresses needs existence of genetic diversity. In this research, reaction of 53 sunflower mutant lines seedlings accompanied by their parental line was studied in several salinity levels including 0, 2, 4, 6, 8 ds/m. Also, in molecular experiment, genetic diversity of mutant lines was inspected using AFLP markers. Results revealed significant differences among the studied lines in each of salinity level based on seedling characteristics. Two hundreds loci were amplified using 17 combinations of AFLP primers in the studied lines. Analysis of population structure classified studied mutant lines in two subgroups. Considering association mapping, 43 AFLP markers had significant relation with seedling characteristics. Phenotypic variance of the identified markers varied between 0.13 and 0.42.

Keywords

20.1001.1.22520783.1394.5.11.5.6

Main Subjects

Genomics

References

Abdollahi Mandoulakani B, Yaniv E, Kalendar R, Raats DH, Bariana S, Bihamta MR, Schulman AH (2015) Development of IRAP‑ and REMAP‑derived SCAR markers for marker‑assisted selection of the stripe rust resistance gene Yr15 derived from wild emmer wheat. Theor. Appl. Genet. 128: 211-219.

Ahloowalia BS, Maluszynski M, Nichterlein K (2004) Global impact of mutation-derived varieties. Euphytica 135: 187-204.

Almansouri M, Kinet JM, Lutts S (2001) Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant Soil 231: 243-254.

Al-Maskri AH, Sajjad M, Khan SH (2012) Association mapping: a step forward to discovering new alleles for crop improvement. Int. J. Agric. Biol. 14: 153-160.

Alvarez A, Fuentes JL, Deus JE, Duque MC, Cornide MT (2000) Genetic diversity analysis in rice mutants using isozyme and morphological markers. Cultivos Tropicales 21: 39-44.

Atak M, Kaya, MD, Kaya G, Cıkılı Y, Ciftçi CY (2006) Effects of NaCl on the germination, seedling growth and water uptake of triticale. Turk. J. Agric. For. 30: 39-47.

Atayee R, Mohammadi V, Talaie A, Naghavi MR (2013) Association mapping of root traits in Barley. Iranian J. Field Crop Sci. 44: 347-357 (In Farsi).

Boyd LA, Smith PH, Wilson AH, Minchin PN (2002) Mutations in wheat showing altered field resistance to yellow and brown rust. Genome 45: 1035-1040

Camargo CEDO, Neto AT, Filho AWPF, Felico, JC (2000) Genetic control of aluminum tolerance in mutant lines of the wheat cultivar Anahuac. Euphytica 114: 47-53.

Chen CL, Wang HH, Jeng TL, Chuang SJ, Wei ML, Min SJ (2011) Genetic diversity in NaN3-induced common bean mutants and commercial varieties detected by AFLP. Crop Breed Appl. Biotech. 11: 365-369.

Dadras AR, Sabouri H, Mohammadi Nejad G, Sabouri A, Shoai-Deylami M (2014) Association analysis, genetic diversity and structure analysis of tobacco based on AFLP markers. Mol. Biol. Rep. 41: 3117-3129.

Darvishzadeh R, Poormohammad Kiani S, Huguet T, Sarrafi A (2008) Genetic variation and identification of molecular marker associated with partial resistance to Phoma macdonaldii in gamma-irradiation-induced mutants of sunflower.Can. J. Plant Pathol. 30: 106-114.

Davar R, Majd A, Darvishzadeh R, Sarrafi A (2011) Mapping quantitative trait loci for seedling vigour and development in sunflower (Helianthus annuus L.) using recombinant inbred line population. Plant Omics J. 4: 418-427

Ebrahimi A, Sarrafi A (2012) Genetic variability and identification of markers associated with germination parameters in gamma-irradiation induced mutants of sunflower under water stress condition. Iranian J. Genet. Plant Breed. 1: 1-8.

Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol. 14: 2611–2620.

Fulton TM, Chunwongse J,TanksleySD(1995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol. Biol. Rep. 13: 207-209.

Hossain MI, Khatun A, Talukder MSA, Dewan MMR, Uddin MS (2010) Effect of drought on physiology and yield contributing characters of sunflower.BangladeshJ. Agric. Res. 1: 113-124.

Hu J, Seiler G, Kole C (2010) Genetics, Genomics and Breeding of Sunflower. Routledge, USA.

Kaya MD, Okçu G, Atak M, Cıkılı Y, Kolsarıcı O (2006) Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. J. Agron. 24: 291-295.

Khan MM, Iqbal MJ, Abbas R (2004) Loss of vigour and viability in aged onion (Allium cepa L.) seeds. Int. J. Agric. Biol. 6: 708-11.

Kuroki M, Saito K, Matsuba S, Yokogami N, Shimizu H, Ando I, Sato Y (2007) A quantitative trait locus for cold tolerance at the booting stage on rice chromosome 8. Theor. Appl. Genet. 115: 593-600.

Lu G, Zhang X, Zou Y, Zou Q, Xiang X, Cao J (2007) Effect of radiation on regeneration of Chinese narcissus and analysis of genetic variation with AFLP and RAPD markers. Plant Cell Tiss. Org. 88: 319-327.

Mackay L, Powell W (2007) Methods for linkage disequilibrium mapping in crops. Trends Plant Sci. 12: 57-63.

Maluszynski M, Nichterlein K, Van Zanten L, Ahloowalia BS (2000) Officially released mutant varieties the FAO/IAEA database. Mut. Breed. 12: 1-84.

Mandel JR, Dechaine JM, Marek LF, Burke JM (2011) Genetic diversity and population structure in cultivated sunflower and a comparison to its wild progenitor, Helianthus annuus L. Theor. Appl. Genet. 123:693–704.

Mandel JR, Milton FE, Donovan LA, Knapp JS, Burke JM (2013) Genetic diversity and population structure in the rare Algodones sunflower (Helianthus niveus ssp. tephrodes). Conserv. Genet. 14: 31–40.

Matthies IE, Hintum T, Weise S, Roder MS (2012) Population structure revealed by different marker types (SSR or DArT) has an impact on the results of genome-wide association mapping in European barley cultivars. Mol. Breed. 30: 951–966.

Mohammadi SA, Prasanna BM (2003) Analysis of genetic diversity in crop plants- salient statistical tools and considerations. Crop Sci. 43: 1235-1248.

OraguzieNC,Wilcox PLRikkerink EHA, De Silva HN (2007) Linkage Disequilibrium, Association Mapping in Plants. Springer.New York.

Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945–959.

Rachid Al-Chaarani G, Gentzbittel L, Huang X, Sarrafi A (2004) Genotypic variation and identification of QTLs for agronomic traits using AFLP and SSR in recombinant inbred lines of sunflower (Helianthus annuus L.). Theor. Appl. Genet. 109: 1353-1360.

Rachid Al-Chaarani G, Gentzbittel L, Wedzony M, Sarrafi A (2005) Identification of QTLs for germination and seedling development in sunflower (Helianthus annuus L.). Plant Sci. 169: 221- 227.

Rashid M, Ren-hu L, Wei J, Yong-han X, Fu-lin W, Yue-zhi T, Jun-mei W, Cheema AA, Jin-qing C, He G (2009) Genomic diversity among Basmati rice (Oryza sativa L) mutants obtained through 60Co gamma radiations using AFLP markers. Afr. J. Biotech. 8: 6777-6783.

Rostami-Ahmadvandi, H, Cheghamirza, K, Kahrizi, D, Bahraminejad, S (2013) Comparison of morpho-agronomic traits versus RAPD and ISSR markers in order to evaluate genetic diversity among Cuminum cyminum L. accessions. Aust. J. Crop Sci. 7(3):361-367.

Sarrafi A, Kayyal H, Rachid Al- Chaarani G, Cantin F, Chaline AS, Durielle AS (2000) Inheritance of organogenesis parameters in cotyledons of sunflower (Helianthus annuus L.). J. Genet. Breed. 54: 227-331.

Sherif F, Khattab S, Ghoname E, Salem N, et al. (2011) Effect of gamma irradiation on enhancement of some economic traits and molecular changes in Hibiscus Sabdariffa L. Life Sci. J. 8: 220-229.

Sofalian O, Mohammadi SA, Aharizad S, Moghaddam M, Shakiba MR (2008) Mapping of QTLs for frost tolerance and heading time using SSR markers in bread wheat. Afr. J. Biotech. 9: 5260-5264.

Sorkheh K, Malysheva-Otto LV, Wirthensohn MG, Tarkesh-Esfahani S, Martínez-Gómez P (2008) Linkage disequilibrium, genetic association mapping and gene localization in crop plants. Genet. Mol. Biol. 31: 805-814.

Tuberosa R, Salvi S, Sanguineti MC, Landi P, Maccaferri M, Conti S (2002) Mapping QTLs regulating morpho-physiological traits and yield in drought-stressed maize: case studies, shortcomings and perspectives. Ann. Bot. 89: 941-963.

Wang ZF, Wang JF, Bao YM, Wu YY, Zhang HS (2011) Quantitative trait loci controlling rice seed germination under salt stress. Euphytica 178: 297-307.

Witkowicz J, Urbanczyk-Wochniak E, Przybecki Z (2003) AFLP marker polymorphism in cucumber (Cucumis sativus L.) Near isogenic lines differing in sex expression. Cell. Mol. Biol. Lett. 8: 375-381.