Expression of CYP genes involved in sesamin biosynthesis pathway in different varieties of sesame seeds (Sesamum indicum L.)

Document Type : Research Paper

Authors

1 M.Sc. in Agricultural Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran.

2 Assistance Professor of Agricultural Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran.

Abstract

Sesame (Sesamum indicum L.) is one of the oldest oilseed crops that has been a main food source providing beneficial nutritional outcomes to human health due to its high contents of antioxidants and secondary metabolites. The importance of sesame arises from presence of antioxidant sesamin. Over recent decades, due to being used in cancer cases, there have been numerous efforts aimed at better understanding of the genes underlying sesamin biosynthesis and increasing their expression. In this research, expression levels of the key genes in sesamin biosynthesis pathway including CYP81Q1, CYP81Q2, CYP81Q3 and C3H genes were measured in 10 sesame cultivars seeds (Yekta, early Palestinian, Naz tak shakheh, Naz chand shakheh, Oltan, Jiroft, Dashtestan 5, Dashtestan 2, Varamin and Karaj 1) by QRT-PCR technique and 18S rRNA gene as reference gene. Our findings showed that the level of CYP81Q1 gene expression was low in Early Palestinian that has low sesamin content. Therefore, this might not be considered as a qualitatively desired oil cultivar compared to others, whereas Yekta had the highest level of CYP81Q1 expression the best varieties for oil production both qualitatively and quantitatively. The highest expression level of CYP81Q2 was observed in Naz chand shakheh, while the lowest CYP81Q2 expression was found in Early Palestinian. Karaj1, Yekta and Dashtestan 5 showed the highest whereas Oltan had the lowest expression level of C3H. Karaj 1, Yekta and Dashtestan 5 were relatively from the late mature group that was reported to have higher contents of oil and sesamin than early ones.

Keywords

Main Subjects

References

Anterola AM, Jeon JH, Davin LB, Lewis NG (2002) Transcriptional control of monolignol biosynthesis in Pinus taeda factors affecting monolignol ratios and carbon allocation in phenylpropanoid metabolism. J. Biol. Chem. 277: 18272-18280.
Dini Torkamani M, Karaptyan Z (2007) The amount and variety of proteins in seeds of ten cultivars of sesame (Sesamum indicum L.). J. Sci. Technol. Agr. Nat. Res. Soil Wat. Sci. 2(40): 225-231.
 
[1]. P-coumarate 3 hydroxylase
Fischer M, Knoll M, Sirim D, Wagner F, Funke S, Pleiss J (2007) The cytochrome P450 engineering database: a navigation and prediction tool for the cytochrome P450 protein family. Bioinformatics 23: 2015-2017.
Fuss E (2003) Lignans in plant cell and organ cultures: an overview. Phytochem. Rev. 2: 307-320.
Hata N, Hayashi Y, Okazawa A, Ono E, Satake H, Kobayashi A (2010) Comparison of sesamin contents and CYP81Q1 gene expressions in
 
     aboveground vegetative organs between two Japanese sesame (Sesamum indicum L.) varieties differing in seed sesamin contents. Plant Sci. 178: 510-516.
Hwan Lee J, Baek IY, Ko JM, Shim KB, Kang NS, Kim HT (2008) Correlation of lignan contents with protein and oil contents in the seeds of Sesamum indicum L. J. Appl. Biol. Chem. 51(1): 20-27.
Hemalatha S (2004) Lignans and tocopherols in Indian sesame cultivars. J. Am. Oil. Chem. Soc. 81: 467-470.
Hirose N, Nishihara K, Sugano M, Akimoto K, Shimizu S,Yamada H (1991) Inhibition of cholesterol absorption and synthesis in rats by sesamin. J. Lipid Res. 32: 629-638.
Jeng KC (2005) Sesamin and sesamolin: Nature’s therapeutic lignans. Curr. Enz. Inhib. 1: 11-20.
Kamal-Eldin A, Frank J, Razdan A, Tengblad S, Basu S, Vessby B (2000) Effects of dietary phenolic compounds on tocopherol, cholesterol, and fatty acids in rats. Lipids 35: 427-435.
Katsuzaki H, Kawasumi M, Kawakishi S, Osawa T (1992) Structure of novel antioxidative lignan glucosides isolated from sesame seed. Biosci. Biotech. Bioch. 56: 2087-2088.
Khodadadi A, Hamidinia M, Boroujerdnia M, Mohammadi J (2011) The impact of real-time PCR method using a standard curve and linear regression approach. J. Jondi Shapour. 11(76): 85-95.
Kilkkinen A, Erlund I, Virtanen MJ, Alfthan G, Ariniemi K, Virtamo J (2006) Serum enterolactone concentration and the risk of coronary heart disease in a case-cohort study of Finnish male smokers. Am. J. Epidemiol. 163: 687-693.
Kuzhuvelil B. Harikumar, Sung B, Tharakan ST, Pandey MK,Joy B,Guha S,Krishnan S,AggarwalBB (2010) Sesamin manifests chemopreventive effects through suppression of NF-κB-regulated cell survival, proliferation, invasion and angiogenic gene products. Mol. Cancer Res. 8: 751-761.
Lim JS, Adachi Y, Takahashi Y, Ide T (2007) Comparative analysis of sesame lignans (sesamin and sesamolin) in affecting hepatic fatty acid metabolism in rats. Brit. J. Nutr. 97: 85-95.
Mansuy D (1998) The great diversity of reactions catalyzed by cytochromes P450. Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol. 121: 5-14.
Miyawaki T, Aono H, Toyoda-Ono Y, Maeda H, Kiso Y, Moriyama K (2009) Antihypertensive effects of sesamin in humans. J. Nutr. Sci. Vitaminol. 55: 87-91.
Moazzami AA, Andersson RE, Kamal-Eldin A (2006) HPLC analysis of sesaminol glucosides in sesame seeds. J. Agr. Food Chem. 54: 633-638.
Mukhopadhyay N, Ray AK (1999) Effect of fermentation on the nutritive value of sesame seed meal in the diets for rohu, Labeo rohita (Hamilton), fingerlings. Aquacult. Nutr. 5 (4): 229-236.
Nakai M, Harada M, Nakahara K, Akimoto K, Shibata H, Miki W, Kiso Y (2003) Novel antioxidative metabolites in rat liver with ingested sesamin. J. Agr. Food Chem. 51: 1666-70.
Nakano D, Itoh C, Ishii F, Kawanishi H, Takaoka M, Kiso Y, Tsuruoka N, Tanaka T, Matsumura Y (2003) Effects of sesamin on aortic oxidative stress and endothelial dysfunction in deoxycorticosterone acetate-salt hypertensive rats. Biol. Pharm. Bull. 26: 1701-1705.
Namiki M (1995) The chemistry and physiological functions of sesame. Food Rev. Int. 11: 281-329.
Ono E, Nakai M, Fukui Y, Tomimori N, Fukuchi-Mizutani M, Saito M, Satake H, Tanaka T, Katsuta M, Umezawa T (2006) Formation of two methylenedioxy bridges by a Sesamum CYP81Q protein yielding a furofuran lignan,(+)-sesamin. Proc. Natl. Acad. Sci. 103: 10116-10121.
Osawa T, Nagata M, Namiki M, Fukuda Y (1985) Sesamolinol, a novel antioxidant isolated from sesame seeds. Agric. Biol. Chem. 49: 3351-3352.
Qusti S, Abo-Khatwa A, Lahwa M (2010) Screening of antioxidant activity and phenolic content of selected food items cited in the Holly Quran. J. Biol. Sci. 2: 40-51.
Rayani A, Dehghan Nayeri F (2015) An improved method for extraction of high-quality total RNA from oil seeds. Biotechnol. Lett. 37: 927-933.
Sansen S, Yano JK, Reynald RL, Schoch GA, Griffin KJ, Stout CD, Johnson EF (2007) Adaptations for the oxidation of polycyclic aromatic hydrocarbons exhibited by the structure of human P450 1A2. J. Biol. Chem. 282: 14348-14355.
Saxena A, Singh P, Yadav DK, Sharma P, Alam S, Khan F, Thul ST, Shukla RK, Gupta V, Sangwan NS (2013) Identification of cytochrome P450 heme motif in plants proteome. Plant Omics J. 6(1): 1-12.
Tsai SC, Liu YC, Li CP, Huang TS, Lee CC (2006) Sesamin inhibits vascular endothelial cell growth and angiogenic activity of lung adenocarcinoma cells. J. Cancer Mol. 2: 199-205.
Wahid OARA, El-Bramawy MAS (2007) Resistance of some sesame (Sesamum indicum L.) collections against root rot disease (Rhizoctonia solani Kühn) under field conditions. J. Plant Prot. Res. 47: 321-327.
Wang L, Li P, Zhang W, Wang X, Qi X, Zhang X, Zhang Y (2013) Variation of sesamin and sesamolin contents in sesame cultivars from China. Pak. J. Bot. 45(1): 177-182.
Wei X, Liu K, Zhang Y, Feng Q, Wang L, Zhao Y, Li D (2015) Genetic discovery for oil production and quality in sesame. Nat. Commun. 6: 8609-8618.
Wong, ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques. 39:1-75.
 
 
 
 
 
 
 
Crop Biotechnology
Volume 5, Issue 2 - Serial Number 12
February 2016
Pages 11-23

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