Identifying QTLs associated with salinity tolerance in the early stages of germination of barley

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Crop Science and Biotechnology, Payame Noor University, Tehran, Iran.

2 M.Sc. Student, Department of Crop Science and Biotechnology, Payame Noor University, Tehran, Iran.

Abstract

Salinity is one of the main osmotic stresses that limit plants growth and development through changes in osmotic and ionic balances. In order to mapping of genomic regions of controlling quantitative trait locus (QTLs), related to salinity tolerance in grain barley and assessment of associated indices, an experiment was conducted during 2015 using 72 double haploid lines of barley along with their parents (Stepto and Morex) in randomized complete block design (RCBD) with two replications, at three conditions including normal and two salinity levels of NaCl (100 and 200 mM NaCl). Investigated traits in this study were coefficient of velocity of germination, final germination percentage, mean germination time, germination rate index and average germination speed. QTL analysis using composite interval mapping (CIM) was performed based on mean of three environments. Overall, for different traits 47 QTL with LOD≥2.5 was identified and 7 additive-by-additive significant epistatic effects were observed. Total phenotypic variance explained by the QTLs varied from 29.97 to 77.15 percent, which the lowest related to germination rate index under stress conditions of 200 mM and the highest related to coefficient of velocity of germination in normal conditions. The highest LOD (8.27) was obtained for coefficient of velocity of germination on chromosome of 4Hb under 100 mM stress conditions. Stable QTLs in three environments as well as linked markers could be used in the marker assisted selection (MAS) to improve germination characteristics under salinity conditions at different trials replicated over years.

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References

Almudaris MA (1998) Notes on various parameters recording the speed of seed germination.  DerTropenlandwirt.  99: 147-154.
Bernestin L (1974) Crop growth and salinity. p.39 - 54.
Blum A (1988) Plant breeding for stress environments. CRC Press. 163-181.
Brem RB, Kruglyak L (2005) The landscape of genetic complexity across 5,700 gene expression traits in yeast. Proc. Natl.  Acad.  Sci. U.S.A. 102: 1572-1577.
Chalish L, Houshmand S (2011) Estimate of Heritability and Relationship of Some Durum Wheat Characters Using Recombinant Inbred lines. EJCP. 4(2): 223-238.
Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica. 142: 169-196.
Collard BCY, Mackill DJ (2008) Marker assisted selection: An approach for precision plant breeding in the twenty first century. Philosophical Transactions of the Royal Society. Biological Sciences. 363: 557-572.
Dadshani SAW, Weidner A, Buck-Sorlin GH, Börner A, Asch F (2004) QTL Analysis for Salt Tolerance in Barley. University of Bonn, Institute of Plant Nutrition, Germany, Institute of Plant Genetics and Crop Plant Research (IPK),Germany, Brandenburgisch-technische Universität (BTU).
 De F, Kar RK (1994) Seed germination and seeding growth of mung been under water stress induced by PEG 6000. Seed Science and Technology. 23: 301-304.
Doerage RW, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics. 142: 285-294.
Ehdaie  B,  Waines  JG  (1989)  Genetic variation, heritability and path analysis in  land  races  of  bread  wheat  from South  Western  Iran. Euphytica. 41: 183-190.
Emebiri L, Michael P, Moody DB, Ogbonnaya FC, Black C (2009) Pyramiding QTLs to improve malting quality in barley: gains in phenotype and genetic diversity. Molecular Breeding. 23: 219-228.
Fakheri, BA, Mehravaran L (2012) Identify QTL controlling agronomic traits in barley doubled haploid population of Steptoe × Morex under drought stress. Plant Science, 47-57.
FAO (2013) FAOSTAT, http://faostat.fao.org/site/.
FAO (2010) Extent and causes of salt–affected soils in participating countries. Available on URL:http://www.fao.org/ag/AGL/agll/spuch/topic4.htm.
Farhoudi R (2013) Investigation the salinity tension effect on growth and physiological characteristics of nine wheat cultivars at vegetative growth stage. Crop Physiology. 5(20): 71-86.
Greenway H, Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Annual Review of Plant Physiology. 31(1): 149-190.
Han F, Ullrich SE, Kleinhofs A, Jones B L, Wesenberg DM (1997) Fine structure mapping of the barley chromosome-1 centromere region containing malting-quality QTLs. Theoretical & Applied Genetics. 95: 903-910.
 Hayes PM, Liu BH, knapp SJ, chen F, Jones B, Blake T, Franckowiak J,Rasmmusson D, Sorrells M, Ullrich SE, Wesenberg D, Kleinhofs A (1993) Quantitative traits locus effects and environmental interaction in a sample of North American barely germplas Theoretical and Applied Genetics. 87: 392-401.
Jafary H, Ansari MN, Ebrahimi MA, Taheri M, Dastkar E (2013) B Analyses of Quantitative Trait Loci Involved in Production of Enzymes Conferring Salt Tolerance in Barley (Hordeum vulgare L.). Crop Biotech. 5: 117-127.
Kafi M (2008) Saline agriculture and its necessity in Iran. In: Proceeding of 10th Iranian Crop Sciences Congress, Karaj, Iran.
Khalili M and Mohammadi S A (2015) Mapping QTLs associated with wheat seed germination under normal and drought stress conditions. Crop Biotech. 9: 1-14.
Khalily M, Tourchi M, Aharizad S, Moghaddam Vahed M, Payghambari SA (2014) Detecting.
QTLs Related to Barley Seed Malt Under Water Deficit Stress. Plant Science. 201-212.
Korff M, Wang H, Le´on J, Pillen K (2008)  AB-QTL  analysis  in  spring barley:  III.  Identification of exotic alleles for the improvement of malting quality. Molecular Breeding. 21: 81-93.
Mano Y, Takeda K (1997) Mapping quantitative trait loci for salt tolerance at germination and the seedling stage in barley (Hordeum vulgare L.). Euphytica. 94: 263-272.
Matus IA, Hayes PM (2002) Genetic diversity in three groups of barley germplasm assessed by simple sequence repeats. Genome. 45: 1095-1106.
Munns R (2002) Comparative physiology of salt and water stress. Plant, Cell and Environment 25, 239-250.
     Sudhir P, Murthy S D S (2004) Effects of salt stress on basic processes of photosynthesis. Photosynthetica. 42(4): 481-486.
Wang S, Basten C J, Zeng Z B (2007) Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, Raleig, NC. (Available at http:// statgen. Ncsu.edu/qtlcart/wQTL.htm/).
Zaare M, Jafari H (2013) Quantitative trait loci diversity for salt tolerance at the early growth stage of barley (Hordeum vulgare L.). Crop Breeding Journal. 3(2): 69-77.
Zhang  ZH,  Yu  SB,  Yu  T (2005)  Mapping quantitative trait loci (QTLs) for seedling Vigor using recombinant inbred lines of rice (Oryza sativa L.). Field Crop Res. 91: 161-170.
Zhu, JK (2001). Plant salt tolerance. Trends in Plant Science. 6(2): 66-71. 

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