Genetic diversity and association analysis for morphophenolgic traits and resistance to Powdery mildew using ISSR, IRAP and iPBS markers

Masoudi, Hooriyeh; Sabouri, Hossein; Taliey, Fakhtak; Jafarby, Jabbar Alat

Genetic diversity and association analysis for morphophenolgic traits and resistance to Powdery mildew using ISSR, IRAP and iPBS markers

Document Type : Research Paper

Authors

¹ M.Sc. Student of Agricultural Biotechnology, Gonbad Kavous University, Gonbad Kavous, Iran

² Assocate Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.

³ Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran.

⁴ Academic member, Seed and Plant Improvement Research Department, Golestan Agricultural and Natural Resources Research and Education Center, AREEO, Gorgan, Iran.

Abstract

In order to evaluation of genetic diversity and association analysis of morpho-phonological traits and mildew disease of wheat germplasm, 115 wheat genotypes were planted in the research field of Gonbad Kavous University as RCBD design with 3 replications in 2015-16. According to the results, there was a significant difference between all measured traits except grain length. In order to evaluation of molecular diversity in 60 wheat genotypes, 8 ISSR, IPBS and IRAP primers were used. Out of 61 bands produced in total, 47 bands were polymorph. The number of polymorphic bands was different range from 2 to 14 bands for each primer. The maximum percent of polymorphism with %100 polymorphism belonged to PRI-46 primer, and the minimum percent of polymorphism with %50 polymorphism belonged to PRI-59, PRI -10 and PRI-5 primers. In this study, also 60 genotypes and lines of wheat were evaluated in-vitro against Bgt. Cluster analysis of data of Powdery mildew caused was grouped lines in three groups of sensitive, resistance and median using UPGMA algorithm and Euclidian distance. Mean comparison using LSD indicated that lines 127 and 801 were high resistance but lines 390, 515 and 833 were susceptible to Bgt. Association analysis between markers and traits morpho-phenological showed that a total of 56 alleles showed a connection between the characters and PR50-6 allele associated most relevant to the characters. Based on results of this research, the higher genotypes could be screened for advanced wheat breeding steps.

Keywords

20.1001.1.22520783.1396.7.18.4.3

Main Subjects

Molecular Plant Breeding

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