Characterization and expression profiling of AlPKL gene in response to salinity stress and recovery conditions in halophyte Aeluropus littoralis

Faraji, Sahar; Hashemi-Petroudi, Seyyed Hamidreza; Najafi-Zarrini, Hamid; Ranjbar, Gholamali

Characterization and expression profiling of AlPKL gene in response to salinity stress and recovery conditions in halophyte Aeluropus littoralis

Document Type : Research Paper

Authors

¹ Former M.Sc. Student of Plant Breeding, Sari Agricultural Science and Natural Resources University, Sari, Iran

² Assistant Professor, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

³ Assistant Professor, Sari Agricultural Science and Natural Resources University, Sari, Iran

⁴ Associate Professor, Sari Agricultural Science and Natural Resources University, Sari, Iran

Abstract

Salinity is considered as a perilous environmental stress reducing crop yields, which makes the plant survive difficult via stopping the various mechanisms of it, eventually leading to death. Genes are the momentous factors in multiple physiological pathways regarding to their involvements in stress responses. The gene encoding for Chromodomain Helicase DNA protein (PICKLE, PKL) is one of them, which regulates the other stress-responsive genes transcriptions under unfavorable conditions. Transcripts assay in halophyte Aeluropus littoralis, as a valuable genetic resource, will provide the inspiring information for sensitive crops improvement. Therefore, biochemical properties, functional domains, phylogenetic analysis and promoter cis-elements were investigated in this study, suggesting that this gene may play the critical roles in dealing with stimulus circumstances. Expression profiling of AlPKL in coping with salinity and recovery situations in A. littoralis shoot and root tissues through the qReal-Time PCR technique was also revealed high transcript magnitudes of this gene. Hence, further studies on PKL genes in multiple plant species can provide precious information for better understanding of stress endurance mechanisms.

Keywords

20.1001.1.22520783.1396.7.20.2.5

Main Subjects

Molecular Plant Breeding

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