Semi quantitative analysis of betaine aldehyde dehydrogenase gene expression in potato plant under salinity stress

Mohammadi, Zhila; Motallebi-Azar, Alireza; Zaree-Nahandi, Fariborz; Tarinejad, Alireza; Gohari, Gholamreza

Semi quantitative analysis of betaine aldehyde dehydrogenase gene expression in potato plant under salinity stress

Document Type : Research Paper

Authors

¹ Former M.Sc. Student of Horticulture, Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.

² Associate Professor, Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.

³ Assistant Professor, Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.

⁴ Assistant Professor, Department of Horticulture, Faculty Agriculture, University of Maragheh, Maragheh, Iran.

Abstract

The present study was aimed to investigate of nitric oxide effect on betaine aldehyde dehydrogenase gene expression and glycine betaine synthesis in Solanum tuberosum cv. Agria under salinity stress on in vitro condition. The experiment treatments included four level of sodium nitroprussideas a nitric oxide donor (0, 10-3, 10-4 and 10-5mM) and two level of sodium chloride (0 and 70 mM). In the present study, MS media culture was used and sodium nitroprusside was applied for increasing the betaine aldehyde dehydrogenase gene expression (the responsible gene of glycine betaine synthesis) under salinity stress. Four weeks after treatment, total RNA of treated explants was extracted and semi quantitative RT-PCR was used for the analysis of expression of betaine aldehyde dehydrogenase gene. The glycinbetaine content was measured with iodide potassium. The survey of betaine aldehyde dehydrogenase gene expression showed that the expression of this gene was increased under salinity stress however, the sodium nitroprusssid decreased its expression under salinity stress. Also sodium nitroprussid increased the glycine betaine content in grown plantlets which were grown under normal condition however under salinity stress this compound showed negative effect on glycinbetaine content.

Keywords

20.1001.1.22520783.1396.7.20.5.8

Main Subjects

Biotic and Abiotic stress

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