Identification of the key salt tolerance involved genes in wheat using microarray data analysis

Amirbakhtiar, Nazanin; Shobbar, Zahra-Sadat; Ismaili, Ahmad; Nazarian Firouzabadi, Farhad; Ghaffari, Mohammad Reza

Identification of the key salt tolerance involved genes in wheat using microarray data analysis

Document Type : Research Paper

Authors

¹ Ph.D. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, IranKhorramabad, Lorestan

² Assistant Professor, Agricultural Biotechnology Research Institute of Iran, Department of Systems Biology, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

³ Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

⁴ Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

Abstract

Salt stress is considered as one of the most important constraints in wheat production worldwide, thus for, research toward development of tolerant varieties is of great importance. Discovering genes and molecular mechanisms involved in salt tolerance are the primary steps in molecular breeding for salinity. In this study, taking advantage of the data deposited in NCBI Gene Bank, two salinity-related microarray data sets of bread wheat were analyzed to identify salt responsive genes. Bioinformatics’ analyses indicated that 3096 and 2060 genes were salt responsive genes in root and shoot, respectively. Gene ontology analysis of salt responsive genes showed that these genes were enriched for response to chemical stimulus, response to oxidative stress, transport, regulation of transcription and carbohydrate metabolic process in biological process category in both tissues. Furthermore, the differentially expressed genes in metabolic process category were enriched for catalytic activity, binding and oxidoreductase activity in both tissues. In order to determine the key genes involved in salt tolerance, hub analysis was performed on the salt responsive genes identified in the root. Based on the achieved results, the role of regulatory genes including protein kinases, protein phosphatases and transcription factors such as MYB and WRKY, was highlighted in inducing salt tolerance.

Keywords

20.1001.1.22520783.1397.8.21.7.9

Main Subjects

Biotic and Abiotic stress

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