Serological and molecular detection of Potato virus S (PVS) from common potato cultivars in Ardabil province

Ehteshm Rasi, Nasim; Rouhibakhsh, Ahmad; Soheili Moghadam, Bita; Masoudi, Nahid

Serological and molecular detection of Potato virus S (PVS) from common potato cultivars in Ardabil province

Document Type : Research Paper

Authors

¹ M.Sc. of Plant Protection, Department of Plant Protection, Faculty of Agricultural Science, Guilan University, Rasht, Iran

² Assistant Professor, Department of Plant Protection, Faculty of Agricultural Science, University of Guilan, Rasht, Iran.

³ Scientific member of Plant Protection Research Department, Ardabil Agricultural and Natural Resources Research and Education Centre (AREEO), Ardabil, Iran

⁴ Ph. D. candidate, Department of Plant Protection, Faculty of Agricultural Science, University of Guilan, Rasht, Iran

Abstract

Potato virus S (PVS) of the genus Carlavirus belongs to the Betaflexiviridae family is one of the important potato infectious viruses. To detect PVS, the suspected leaf samples were collected from potato fields in Ardabil province during summer in 2015 and 2016. To assess the biological properties, symptomatic leaves inoculated to indicator plants in the greenhouse condition. The samples were tested for PVS infection using DAS-ELISA. RT-PCR for molecular detection was done using specific primers related to the coat protein area of Potato virus S. The coat protein gene nucleotide sequences of two isolates of Potato virus S called Ag-9 and Os-11 obtained from Agria and Oceana cultivars were determined. The CP sequences of two isolates were compared with each other and with 29 other isolates of the virus. The reconstructed phylogenetic tree clustered the isolates in two main groups I (subgroups IA and IB) and II. Two Ag-9-PVS-F and Os-11-PVS-F isolates were clustered in IB subgroup along with isolates from Azarbaijan, Hamedan, Kerman and Esfahan as well as isolates from Hungary, Ukraine, Scotland and India. The putative coat protein amino acid sequence alignment of this two isolates with 6 Iranian and 5 foreigner isolates showed difference in 8 and 25 amino acids for Ag-9-PVS-F and Os-11-PVS-F isolates respectively. The results indicate that there are various PVS isolates in the country, and the difference in nucleotide and amino acid sequences may indicate differences in the geographic origin and hence the type and timing of entry of virus isolates into the region.

Keywords

20.1001.1.22520783.1397.8.22.5.9

Main Subjects

Molecular farming

References

Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2011) Family Betaflexiviridae. Pages 920-941 in: Virus taxonomy: Ninth report of the international committee on taxonomy of viruses. King AMQ Adams MJ, Carstens EB, Lefkowitz EJ, eds. Elsevier, Academic Press, San Diego, CA.

Ahmadi K, Gholizadeh H, Ebadzadeh H, Hatami F, Hossein Pour R, Kazemi Fard R, Abdshah H (1394) Agricultural statistics of the year of 2014-2015, Vol. 1: Crop products. Ministry of Agriculture Press-ICT Center, First Edition, 163 pp.

Andreeva L, Jarvekulg L, Rabenstein F, Torrance L, Harrison BD, Saarma, M (1994) Antigenic analysis of potato virus A particles and coat protein. Ann. Appl. Biol. 125: 337-348.

Clark MF, Adam AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay (ELISA) for the detection of plant viruses, J. Gen. Virol. 34: 475.

Danesh D, Soleimanian S, Philosoph F Dehghan M, (1992) The frequency of four potato viruses in experimental field of Friedan of Isfahan, Plant disease, 28: 1-9.

De Bokx J A (1970) Reactions of various plant species to inoculation with Potato virus S, Neth. J. Plant Path. 76: 70-78.

De Bokx, JA Van der Want JPH (1987) Viruses of potato and seed-potato production, 2^nd Ed. Center for Agricultural Publishing and Documentation, Pudoc., Wageningen, Netherlands, 259 pp.‏

De Bruyn Ouboter MP (1952) A new potato virus. In: Proc. Conf. on Potato Virus Diseases. Wageningen-Lisse, The Netherlands, pp. 83-84.

Hassan Panah D, Nick Shad KH, Hassni M (2008) Seed potato production, Ardabil Province Agricultural Jihad Organization, 193 pp.

Hosseini F, Pourrahim R, Maleki M, Farzadfar Sh (2013) Incidence, biological properties and phylogenetic status of Potato virus A Isolates in Khorasan-e-Razavi and Markazi provinces based on coat protein gene. Crop Biotech. 4: 131-139.

Hull R (2014) Plant virology (5th Ed.), Academic Press, New York, 1104 pp.

Khakvar R, Pourrahim R, Shamsbakhsh M (2000) Study on six potato viruses in Khuzestan provience, in Proceedings of the 14^th Iranian Plant Protection Congress, Tehran, Iran, 312 pp.

Kerlan C (2008) Potato viruses. In: Mahy BWJ Regenmortel MHV (Eds). (2010) Desk enciclopedia of plant and fungal virology. San Diego, California, USA pp 458- 470.

Kumar S, Stecher G, Tamura K (2016) Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. and Evol. 33:1870-1874

Lambert SJ, Scott JB, Pethybridge SJ, Hay FS (2012) Strain characterization of Potato virus S isolates from Tasmania, Australia, Plant Dis. 96: 813-819.

Lin YH, Druffel K L, Whitworth J, Pavek M J, Pappu HR (2009) Molecular characterization of two potato virus S isolates from late-blight-resistant genotypes of potato (Solanum tuberosum), Arch. Virol. 154: 1861-1863.

Loebenstein G, Berger PH, Brunt A, Lawson RH (2001) Virus and Virus Like Diseases of Potatoes and Production of Seed Potatoes, Kluwer Academic Publishers, 460 pp.

Matoušek J, Schubert J, Dědič P, Ptáček J (2000) A broad variability of potato virus S (PVS) revealed by analysis of virus sequences amplified by reverse transcriptase-polymerase chain reaction, Can. J. Plant Pathol. 22: 29-37.

Matousek J, Schubert J, Ptacek J, Kozlova P, Dedic P (2005) Complete nucleotide sequence and molecular probing of potato virus S genome, Acta Virol. 49: 195–205.

Mossahebi GHM (1996) In Proceedings of the 1^st Iranian Horticultural Congress, Iran, p. 79.

Mulholland V (2005) Immunocapture-polymerase chain reaction in immunochemical protocols, 3rd edition, Human press, Totowa, USA, pp: 281-290.

Nyalugwe EP, Wilson CR, Coutts BA, Jones RAC (2012) Biological properties of Potato virus X in potato: effects of mixed infection with Potato virus S and resistance phenotypes in cultivars from three continents, Plant Dis. 96: 43-54.

Pourrahim R, Farzadfar Sh, Golnaraghi AR, Ahoonmanesh A (2007) Incidence and important of viral pathogens in some Iranian potato fields, Plant Dis. 91: 609-615.

Rohani H, Taleb-Zadeh F (1974) In Proceedings of the 15^th Plant Medical Congress, Tabriz, Iran, p. 64.

Salari KH, Massumi H, Shabanian M, Heydarnejad J, Hosseini Pour A (2006) In Proceedings of the 16th Iranian Plant Protection Congress, Tehran, Iran, p. 320.

Salari KH, Massumi H, Heydarnejad J, Hosseini Pour A, Varsani A (2011) Analysis of Iranian Potato virus S isolates, Virus Genes, 43: 281-288.

Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucleic Acids Res. 22: 4073-4080.

Wale S, Platt HW, Cattlin N (2008) Diseases, Pests and Disorders of Potatoes, a Colour Handbook. Manson Publishing Ltd. UK. 176 pp.