Saturation of genetic map and locating of (QTLs) related to traits in barley using F3 families caused Badia× Kavir cross

Bakhtiari, Samira; Sabouri, Hossein; Mollashahi, Mehdi; Hossein Moghaddam, Hossein

doi:10.30473/cb.2019.5411

Saturation of genetic map and locating of (QTLs) related to traits in barley using F3 families caused Badia× Kavir cross

Document Type : Research Paper

Authors

¹ M.Sc. in Agricultural Biotechnology, University of Gonbad-Kavoos, Iran.

² Associate Professor, Department of Plant Production, University of Gonbad-Kavoos, Iran.

³ Assistant Professor, Department of Plant Production, University of Gonbad-Kavoos, Iran.

10.30473/cb.2019.5411

Abstract

Barley (Hordeum vulgare L.) is a model plant for genetical and physiological studies and has a high adaptability to different conditions. In order to locate QTL, the genomic areas controlling barley agronomic traits under experimental conditions were conducted in 104 families with their parents (Badia and Kavir) in the Research Farm of Gonbad-e-Kavous College in 2013-2014. Agronomic traits such as biomass, spike weight, spike number, spike length, grain yield, peduncle length, stem diameter, flag leaf length, flag leaf width, flag leaf weight, grain numbers, grain weight, and awn length for all families were measured. For the saturation of map, 19 (93 alleles), the ISSR marker used that assigned to 7 groups of attachment to 7 chromosomes with a genome length of 617.5 cM and the average between markers equal to 5.41 cM Morgan. A total of 21 locations with QTL were identified for the agronomic traits. Thirteen major effect QTLs that controlled a large proportion of phenotypic variation were identified. Major QTLs The effects of controlling the desired attributes and their linked markers can be used in selection programs using the marker.

Keywords

20.1001.1.22520783.1397.8.24.5.3

Main Subjects

Molecular Plant Breeding

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