Cloning and characterization of a constitutive promoter of polyubiquitin gene from Cicer ariethinum

Document Type : Research Paper

Authors

1 MSc student, Department of Agricultural Biotechnology, Shahed University, Tehran, Iran

2 Assistant professor, Department of Genetic Engineering and Biosafety, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization, Karaj, Tehran, Iran.

Abstract

Producing transgenic plants need new promoters. Due to the advent of next generation sequencing technologies and the production of massive genomic data for various crop plant species paralleled by the development of bioinformatics tools, there is an opportunity ‎to identify, isolate and characterize new promoters. Because of public concern about using viral promoters, there is a need for cloning and using plant promoters in transgenic food crops. Native plant constitutive promoters may be composed of non-specific elements that are simply more efficient at protein recruitment for transcription. Promoters are classified as inducible, constitutive and tissue specific according to the nature of gene expression they regulate. Housekeeping genes are the best and most important sources for isolating the constitutive promoters. In this study, -Glucoronidase reporter gene expression mediated by a polyubiquitin promoter (CaUBQ10) from chickpea (Cicer ariethinum) was analyzed in tobacco tissues. The functionality of the CaUBQ10 was confirmed in leaves, stems, and roots of stably transformed tobacco plants and suggested that the CaUBQ10 is a constitutive promoter and may provide a valuable choice for high-level expression of target genes during the life cycle of a plant.

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References

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