Comparative analysis of primary metabolites to identify regulatory metabolic networks involved in blue color formation in Hydrangea macrophylla

Document Type : Research Paper

Authors

1 Ph.D. Candidate of Plant Physiology, Department of Biology, Payame Noor University, Tehran, Iran.

2 Associate Professor, Department of Biotechnology, Payame Noor University, Tehran, Iran.

3 Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran.

4 Professor, Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization, Karaj, Tehran, Iran.

5 Assistant Professor, Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education, and Extension Organization, Karaj, Tehran, Iran.

Abstract

Hydrangea macrophylla is a plant that its blooms turn from pink to blue in the presence of aluminum (Al) and thereby could be considered as a model plant for studying blue color formation. In this study, the metabolite profiling analysis has been performed using ion chromatography coupled to mass spectrometry (IC-MS/MS) and Ultra Performance Liquid Chromatography (UPLC) to investigate the regulatory connected network among metabolites during color turning from pink to blue in full bloom of H. macrophylla in the presence and absence of aluminum. The metabolite profiles resulted in identification of 35 metabolites including two soluble sugars, six sugar phosphates, two sugar nucleotides, four organic acids, four nucleotides and 17 amino acids. Further, a coordinated change was found in glycolytic metabolites showing changes in flux through a pathway during color formation. Moreover, there was a strong correlation among nitrogenous compounds including glutamine, aspartate, glutamate, glycine and threonine indicating the important role of nitrogen metabolism during blue color formation. These findings will facilitate comprehensive research on the regulatory networks of color change in full bloom in H. macrophylla.

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References

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