Isolation and molecular identification of isolates of plant growth promoting Pseudomonas and Bacillus

Document Type : Research Paper

Authors

1 Department of Microbiology, College of basic sciences, Karaj branch, Islamic Azad university, Karaj, Alborz, Iran

2 Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Biology, Payame Noor University, Tehran, Iran

Abstract

The use of the synergistic effect of nitrogen-fixing bacteria and phosphate solubilizing bacteria is one of the newest biofertilizer production strategies. Rhizosphere soil samples were collected from tomato, cucumber and bell pepper commercial greenhouses and screened for Pseudomonas and Bacillus using selective media. One hundred and eight Pseudomonas and 92 Bacillus isolates with PGP activities were isolated and characterized. Fifty-three percent of Pseudomonas isolates and 25% of Bacillus were able to grow on N free medium. Ninety-six percent of Pseudomonas isolates were able to solubilize organic phosphate and 70% of them produce IAA, while none of the Bacillus had these two abilities. About 30% of Pseudomonas and Bacillus isolates produces siderophore, although the ratio of orange halo diameter/colony diameter was greater for Pseudomonas. Only five Bacillus and 31 Pseudomonas isolates inhibited the growth of Phytophthora melonis. Based on these results, although PGP isolates of both genera were found in the rhizosphere soil of commercial greenhouses, these bacteria differed in PGP traits. A Pseudomonas isolate, P3-57 and a Bacillus isolate, C1BY-1 with 50% antagonistic activity against P. melonis which differed in PGP traits selected for future study in greenhouse conditions. Phylogenetic analyses of 16S rRNA gene sequence revealed that strains P3-57 and C1BY-1 are closely related to P. putida and B. subtilis respectively.

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References

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Crop Biotechnology
Volume 8, Issue 2 - Serial Number 24
December 2018
Pages 95-109

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