Genetic diversity and identification of informative microsatellite alleles in rice seedlings under normal conditions and salinity stress

Document Type : Research Paper

Authors

1 Graduate Student, Department of Plant Production, Collage of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran.

2 Associate Professor, Department of Plant Production, Collage of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran.

3 Assistant Professor, Department of Plant Production, Collage of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran.

4 Instructor, Department of Basic Sciences and Technology Engineering, University of Gonbad Kavous, Gonbad Kavos, Gonbad Kavous, Iran.

Abstract

Genetic diversity is of great importance for breeding programs. In order to study the genetic diversity of 102 different rice genotypes based on seedling traits and molecular experiments, an experiment was conducted in a Completely Randomized Design with three replications. The studied traits including Plumule length, radicle length, length of the largest leaf, width of the largest leaf, number of root, fresh shoot weight, fresh weight of root and root volume, and dry weight of shoot, dry weight of root were measured. The average of the Polymorphic of information content (PIC) was estimated to be 0.2716, indicating RM1029 with the of least 0.175 and the RM216 with the highest of 0.435 (PIC). The results of Association analysis between microsatellite marker and seeding traits at normal condition indicated that the RM60B allele for root length, RM127A allele for root, RM231G allele for the fresh weight of the stem explained high percentage of variations. In saline condition, RM129H allele for stem length trait, RM12091 B allele for root attribute, RM263G allele for stem fresh weight, RM127C allele for width of largest leaf explained high percentage of phenotypic variations and were identified as important markers. The results of this experiment can be used in breeding programs.

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Main Subjects

References

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