The effect of ultrasonic stress on the amount of total phenolic content and antioxidant activity in the Securigera Securidaca L. leaves and callus

Document Type : Research Paper

Authors

1 Ph.D Student, Department of Agricultural Biotechnology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

2 Assistant Professor of Plant Physiology and Genetics, Hakim Sabzevari University, Sabzevar, Iran.

3 Associate Professor of Biochemistry, Hakim Sabzevari University, Sabzevar, Iran.

4 Associate Professor, Department of Agronomy, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

Abstract

Abiotic stress and callus formation can increase total phenolic content and antioxidant activity. The aim of this study was to increase the amount of total phenol and antioxidant activity in Securigera securidaca L. and its calli by ultrasonic stress in vitro conditions. The plants were grown in the greenhouse. Callus formation was performed with MS culture medium containing α-naphthalene acetic acid (2.2 mg/L) and 6-Benzylaminopurine (8.8 mg/L). Ultrasonic stress was applied to the main plants for 10, 20 and 30 minutes in 15 days in vitro conditions; in addition, the ultrasonic stress was applied to the calli for 10 minutes at the same time. The samples were collected on the first, fifth, tenth and fifteenth days. The samples were then dried in an oven, then their ethanolic extract was prepared. Total phenol content and antioxidant activity tests were performed by Folin-Ciocalteu and Wu methods respectively on the leaves of the main plant and its calli. The results showed that the amount of total phenol in the 30 minutes on the first day of ultrasonic treatment is higher than the control. In all treatments, antioxidant activity was reduced compared to the control sample. The highest amount of phenol was observed in calli in ultrasonic stress treatment on the 15th day. Antioxidant activity was reduced in calli under ultrasonic stress compared to the control sample. Therefore, it can be concluded that ultrasonic stress in the samples of this experiment increased the amount of phenolic but did not affect the antioxidant activity. Callus formation and ultrasonic stress also increase total phenol content simultaneously.

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References

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