Functional Analysis of AtPHT1;1 promoter in rapeseed (Brassica napus L.) heterologous system

Fathi, Zahra; Zamani, Katayoun; Khosravi, Solmaz; Malboobi, Mohammad

doi:10.30473/cb.2022.63987.1879

Functional Analysis of AtPHT1;1 promoter in rapeseed (Brassica napus L.) heterologous system

Document Type : Research Paper

Authors

¹ Ph.D. Student, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Tehran, I.R. Iran.

² Assistant Professor, Department of Genetic Engineering and Biosafety, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization, Karaj, Iran.

³ Ph.D, Leibniz Institute of Plant Genetics and Crop Plant Research, IPK Gatersleben, Germany.

⁴ Professor, National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Tehran, I.R. Iran.

10.30473/cb.2022.63987.1879

Abstract

Breeding crops with a higher ability in using soil minerals is one of the biotechnology researchers’ goals. Genetic engineering methods provide considerable advances in crop breeding by transferring and creating desired traits for further production under normal or stress conditions. In these procedures, Design of efficient gene constructs is of particular importance and requires promoters with proper function to specifically express the gene of interest in the target tissue and at the appropriate time to develop desired traits such as tolerance to biotic and abiotic stresses or other aims. Specific expression of phosphate-transporter genes in the roots and their induced levels in phosphate deficiency shows the potential of this gene-family promoters utilization in transgenic plants, particularly for the use in phosphate absorption from soil. Bioinformatics analysis showed that the 1826-bp promoter fragment of AtPHT1;1 gene carries several motifs leading to root-specific expression in Arabidopsis thaliana. The expression of a secretory acid phosphatase gene, AtPAP17, as a reporter gene in rapeseed transgenic plants indicated that the AtPHT1;1 promoter retains its root-specific criteria in rapeseed such that it could be used as a regulatory region for the specific expression of desired genes in transgenic rapeseed plant roots.

Keywords

Main Subjects

Molecular Genetics and Genetic Engineering

References

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